逍遥散对肝郁脾虚证模型大鼠海马CA1区和杏仁核BLA区GluR2阳性细胞数变化的影响

Possible regulation channel of Xiaoyao San in treating rats of syndrome of liver invading the spleen by immunohistochemistry

目的:考察逍遥散和CNQX(6-氰基-7-nitroquinoxa-line-2,3-二酮,AMPA受体的拮抗剂)分别对肝郁脾虚证模型大鼠海马CA1区和杏仁核BLA区GluR2阳性细胞数变化的影响。方法:75只SD雄性大鼠随机等分为正常组、模型组、假手术组、CNQX组和逍遥散组。以21d慢性束缚应激造肝郁脾虚证模型组,在此基础上,运用脑立体定位仪微量注射造CNQX组,除逍遥散组灌胃逍遥散悬液外,其余组均给予相应生理盐水。比较各组海马CA1区和杏仁核BLA区GluR2阳性细胞数变化趋势是否一致。结果:在海马CA1区,正常组、CNQX组和逍遥散组间GluR2阳性细胞数变化均无统计学意义;在杏仁核BLA区,CNQX组由于拮抗AMPA受体,GluR2阳性细胞数最少。其它各组变化趋势与CA1区各组间变化趋势相反,且正常组和逍遥散组比较差异无统计学意义。排除了手术创伤等混杂因子,在海马CA1区和杏仁核BLA区,逍遥散组和CNQX组GluR2阳性细胞数变化趋势均相似。结论:确证逍遥散有1条调节通路和CNQX作用机制相似,通过纠正杏仁核和海马的"兴奋-抑制"失衡,重建稳态,来治疗肝郁脾虚证。

Objective： Pervious RT-PCR results revealed the possibility of effective regulation channel of Xiaoyao San in treating rats of syndrome of liver invading the spleen. This experiment was designed to explore CIS rats’ immunohistochemistry findings in hippocampal subregion CA1 and amygdaloid subregion BLA after suppressing both amygdaloid AMPA receptors by microinjection of CNQX. To compare Xiaoyao San group with CNQX group, and to analysis of these findings may prove the possibility of effective regulation channel of Xiaoyao san treating this syndrome. Methods： 75 male SD rats were randomly divided into 5 equal groups： normal group, CIS group, sham-operation group, CNQX group and Xiaoyao San group. The number of GluR2 positive cells in hippocampal subregion CA1 and amygdaloid subregion BLA in these 5 groups were examined by immunohistochemistry test. Results： Compared with control group, the number of GluR2 positive cells decreased in hippocampal subregion CA1. Statistical analysis showed that no difference existed in control, CNQX and Xiaoyao San groups in CA1 region. Except CNQX group, tendency of changes were contradictory in BLA and CA1 region. In addition, the least in BLA in CNQX group. This finding revealed that Xiaoyao San may act the same as CNQX, thus Xiaoyao San afforded effective protection against this depression syndrome through suppression of both amygdaloid excitability. Conclusion： The balance coordination between hippocampus and amygdala may be the key in transformation from adaption to damage in a state of stress, was one of the central mechanisms of liver governing smoothing qi flow （LGSQF） on regulating the stress. Xiaoyao San may effectively regulate the balance of the excitability of hippocampus and amygdala. This hypothesis may reveal the regulation channel on Xiaoyao San affording effective protection against liver invading the spleen syndrome.