电针大陵穴对室性心动过速大鼠心率和血管紧张素Ⅱ含量的影响

Influence of EA on ‘daling’(PC7) on VT rats’ heart rate and contents of Ang Ⅱ in blood plasma

目的:探讨针刺"大陵"穴对室性心动过速(CVT)的调节机制。方法:将40只清洁级SD大鼠,随机分为正常组、模型组、治疗组、对照组,每组10只,采用股静脉插管注射氯化铯的方法,造成大鼠室性心动过速的模型,观察大鼠肢体Ⅱ导联心电图(ECG)的变化,记录心率,治疗组电针大陵穴,对照组电针太渊穴,电针时间为5min,采用放射免疫法检测血管紧张素Ⅱ含量。结果:模型组大鼠心率和血浆血管紧张素Ⅱ含量明显升高,分别为(542±25)次/min,(353.21±49.12)pg/mL,电针大陵穴后大鼠心率和血管紧张素Ⅱ含量向正常状态恢复,分别为(474±25)次/min,(268.44±47.49)pg/mL,电针太渊穴效果不明显。结论:电针大陵穴可促使VT大鼠心率向正常状态恢复;心律失常可能和血管紧张素Ⅱ含量有关,血管紧张素Ⅱ在电针对室性心动过速的调节中有重要作用。

Objective： To observe the changes of heart rate and the contents of angiotensin Ⅱ（AngⅡ） in blood plasma in ventricular tachycardia（VT） rats with electro-acupuncture（EA） on ‘daling’（PC7）. Methods： 40 SD rats were randomly divided into normal, model, treatment and control groups with 10 cases in each. VT model was duplicated by inject CsCl in femoral vein. To observe the rats’ electrocardiogram （ECG） and record their heart rates. EA was applied to‘daling’ （PC7） on treatment group and applied to‘Taiyuan’ （LU9） on control group for 5 minutes. Then, we detected the contents of AngⅡ in the rats’blood plasma respectively. Results： The heart rate and contents of AngⅡin rat increased obviously in model group were （547±30） time/min and （353.21±49.12）pg/mL）. They restored to the normal state after EA ‘daling’ （PC7） are（474±25）time/min and（268.44±47.49）pg/ mL.But the effect was not obvious in ‘Taiyuan’ （LU9）. Conclusion VT rat heart can be prompted to restore to the normal state after EA ‘daling’（PC7）; AngⅡ played an important role in VT.