痹祺胶囊含药血清对体外培养软骨细胞的保护作用

Protective effect of the drug-containing serum with Biqi Capsule on articular chondrocytes in vitro

目的:体外培养脂多糖诱导的关节软骨细胞炎症损伤模型,研究痹祺胶囊(BQC)含药血清对关节软骨细胞的保护作用。方法:制作高、中、低剂量BQC含药血清;以脂多糖刺激软骨细胞诱导炎症损伤模型;MTT法检测不同浓度BQC含药血清对正常培养细胞活力的影响;以不同浓度BQC含药血清干预造模后或未造模的软骨细胞,并设立硫酸氨基葡萄糖(GS)组、不含药的胎牛血清组以及兔血清组以对照,检测各组细胞培养液上清中的一氧化氮(NO)释放量、丙二醛(MDA)产生量,以及超氧化物歧化酶(SOD)活性。结果:BQC高剂量组和GS组均可一定程度提高软骨细胞活性(P<0.01);中、高剂量BQC对LPS诱导释放的NO具有一定程度的抑制作用(P<0.01),且呈一定的剂量依赖性;BQC中、高剂量组MDA产量较造模后的其他各组均有一定程度下调,同时上调SOD活性(P<0.01),且呈一定的剂量依赖性。结论:BQC对抗LPS诱导的脂质过氧化及炎性损伤,对软骨细胞具有较好的保护作用。

Objective： To observe the protective effects of the drug-containing serum with Biqi Capsule （BQC） on articular chondrocytes in vitro by serologic pharmacological test. Methods： Give rabbits Biqi Capsule of three concentrations, then drug-containing serums with BQC are collected. Articular chondrocytes of rabbits are collected and cultured aseptically. The inflammatory responses in chondrocytes are induced by lipopolysaccharide （LPS）. Give a MTT assay for cell proliferation cultured in the nutrient medium with BQC of three concentrations. Treat the LPS induced inflammatory responses in chondrocytes with or without BQC of high, normal, and low concentrations, with Glucosamine sulfate （GS） control group set, then the NO and MDA content in the nutrient solution, and the SOD activity are measured. Results： LPS promotes, significantly compared with the FBS group and rabbit serum group （P〈0.01）, the NO products of chondrocytes. BQC of high concentration and of GS promote the relative activity to a certain degree （P〈0.01）, and the cell proliferation MTT assays not statistically significant between groups treated with/without BQC of the other two concentrations. Either BQC of high concentration or normal inhibits the NO and MDA delivery induced by LPS to a certain degree （P〈0.01）, with a dose dependent. GS also reduces production of NO （P〈0.01）, on a inhibit inhibition level between BQC of high concentration and normal’s. Either BQC of high concentration or normal promotes the SOD activity significantly compared with other groups induced by LPS （P〈0.01）, with a dose dependent. Conclusion： LPS damages articular chondrocytes in vitro by increasing the level of NO and oxyradical. Biqi Capsule antagonizes the lipid peroxidation and inflammatory response induced by lipopolysaccharide in articular chondrocytes, heightens the SOD activity ofchondrocytes and oxyradical clearance, and protects the chondrocytes against LPS induced inflammatory damage.