健骨颗粒含药血清对成骨细胞增殖的影响

Effects of Jiangu Granula Contained Serum on the Proliferation of Osteoblasts

目的:探讨健骨颗粒含药血清对体外培养大鼠成骨细胞增殖的影响。方法:采用酶消化法培养SD大鼠成骨细胞,健骨颗粒含药血清干预,以生理盐水大鼠血清为对照,运用MTT法、流式细胞术检测成骨细胞增殖情况及细胞周期分布。结果:与同浓度生理盐水血清相比,20%健骨颗粒含药血清能明显促进体外培养成骨细胞增殖(P<0.05),明显降低G0/G1期细胞分数(P<0.01或P<0.05),提高S期、G2/M期细胞分数和增殖指数(P<0.01或P<0.05)。结论:健骨颗粒含药血清能推进体外培养成骨细胞细胞周期,从而促进成骨细胞增殖。

Objective：To investigate the effects of Jiangu granula（JGG）contained serum on osteoblast（OB） proliferation of rat in vitro.Methods：Primary cells were isolated from calvaria of newborn SD rats by enzymatic digestion and serial subcultivated.The serum containing JGG or NS were respectively added and cultured the cells.Then Cell proliferation was measured by MTT Method,while the cell cycle were analyzed by flow cytometry（FCM）.Results：Compared with the control group,medium with 20% serum containing JGG significantly stimulated OB proliferation（P〈0.05）,and decreased the cell percentage of G0/G1 phase（P〈0.01 or P〈0.05）,while raised that of S and G2/M phases and proliferation index（PI）（P〈0.01 or P〈0.05）.Conclusion：Jiangu granula contained serum can accelerate the proceeding cell generation cycle,so that to stimulate cell proliferation of OB.