利用Ti质粒介导的二元载体法向小麦导入几丁质酶基因的初步研究

TRANSFERRING CHITINASE GENE INTO WHEATWITH TI-PLASMID BINARY VECTOR SYSTEM

以小麦ＮＰＦＤ、９４－１６７、鄂恩一号３个品种的幼胚、幼穗为受体材料，利用Ｔｉ质粒介导的二元载体法，将含有几丁质酶基因的质粒ｐＢｃｈ导入小麦，经过抗性筛选，ＰＣＲ检测，获得一株转基因小麦，证实了外源几丁质酶基因已整合到小麦基因组中．

The chitinase gene in plasmid,pBch,had been transferred into the immature embryos and point-stems of three wheat cultivars by binary vector system of Ti plasmid.By selection for resistance to kanamycin and PCR assay,it was shown that the alien chitinase gene has been transferred and integrated into the wheat genome.