摘要
以小麦NPFD、94-167、鄂恩一号3个品种的幼胚、幼穗为受体材料,利用Ti质粒介导的二元载体法,将含有几丁质酶基因的质粒pBch导入小麦,经过抗性筛选,PCR检测,获得一株转基因小麦,证实了外源几丁质酶基因已整合到小麦基因组中.
The chitinase gene in plasmid,pBch,had been transferred into the immature embryos and point-stems of three wheat cultivars by binary vector system of Ti plasmid.By selection for resistance to kanamycin and PCR assay,it was shown that the alien chitinase gene has been transferred and integrated into the wheat genome.
出处
《哈尔滨师范大学自然科学学报》
CAS
1999年第2期85-89,共5页
Natural Science Journal of Harbin Normal University