摘要
用固相合成法合成了两种不同链长的含RGD序列的小分子多肽,分别为RGD和GGGGRGDS。用物理共混法制备了含RGD的SS-PEI/DNA复合物。测定了该复合物的电位,研究了RGD的链长和加入量对SS-PEI/DNA复合物在COS-7和CHO中的转染效率的影响。结果表明,由于RGD上的天冬氨酸带负电荷,因此含RGD的复合物电位略有降低。RGD链长对复合物在两种细胞系中的转染影响不大,在COS-7中RGD的转染效率略高于GGGGRGDS,而在CHO中则相反,且随着多肽用量的增加转染效率略有降低。
Two RGD peptides with different chain lengths,RGD and GGGGRGDS,were synthesized via solid-phase synthesis.In order to enhance biological activities,the RGD peptides were noncovalently introduced into SS-PEI/DNA complex.The effects of RGD chain length and addition on zeta potential and transfection efficiency were investigated.The results showed that aspartic acid in RGD carried negative charges could reduce the zeta potential of complex.RGD chain length had slight influence on transfection efficiency.Complex with RGD presented higher transfection efficiency than complex with GGGGRGDS in COS-7 cells,which was different from that in CHO cells.Moreover,transfection efficiency was slightly decreased with the increasing of peptide addition.
基金
高等学校博士学科点专项科研基金资助项目(2006048600)
