红假单胞菌产类胡萝卜素条件的研究

STUDY ON RHODOPSEUDOMONAS PRODUCTION CONDITIONS OF CAROTENOIDS

通过对影响红假单胞菌产类胡萝卜素的因素的全面研究，获得了类胡萝卜素产量相对较高的培养条件，以乙酸钠为碳源，谷氨酸钠为氮源，酵母膏为生长因子，４ｍｇ／ＬＭｇＳＯ４，２ｍｇ／ＬＦｅ２（ＳＯ４）３，１５００ｌｘ光照度，３０℃，微好氧培养１２０ｈ．在此条件下Ｒ－４１菌株的类胡萝卜素产量由２３．８ｍｇ／ｇ干菌体提高到５７．３ｍｇ／ｇ干菌体．

A strain of Rhodopseudomonas sp. R-41, which contains high content of carotenoids was experimented by many factors. The optimum conditions under which the yield of carotenoids is relatively high were found out. That is, using acetate as carbon source, sodium glutamate as nitrogen source, yeast extracts as growth factors, and Mg 2+ 4 mg/L, Fe 3+ 2 mg/L, 1500 lx and incubation in 30 ℃, micro_acerbic for 120 hours. Under the conditions above, the yield of carotenoids increase from 23.8 mg/g drycell to 57.3 mg/g drycell.