摘要
用免疫组织化学方法研究了大白鼠肝脏在４６℃热休克３０ｍｉｎ后恢复期（２．５～７２ｈｒ）内ＨＳＰ７０的表达定位和动态变化及不同温度（４２、４４和４６℃）处理３０ｍｉｎ、恢复１２ｈｒ后ＨＳＰ７０的表达。结果表明：（１）ＨＳＰ７０主要定位于肝细胞的细胞质中；（２）在肝小叶中，阳性细胞多集中于中央静脉周围；（３）４６℃处理３０ｍｉｎ、恢复８～１２ｈｒ，ＨＳＰ７０表达量最多；（４）不同温度热休克处理３０ｍｉｎ、恢复１２ｈｒ表明，４６℃处理组诱导大白鼠肝细胞表达ＨＳＰ７０最多。用体视学方法对实验组的ＨＳＰ７０表达进行了定量组织学分析。
The location and dynamics of HSP70 expression in rat hepar was studied with immunohistochemistry, which were treated under different temperature (42、44、46℃) for 30min and different recovery time (2.5～72hr). The results show: (1)In a hepatic lobule, HSP70 locats in cell plasma; (2)HSP70 is easy to express in the cells nearby centrul vein; (3)Recover for 8-12hr after treatment of 46℃ for 30min, the synthesis of HSP70 reaches climax; (4)The results of heat shock under different temperature (42、44、46℃ for 30min) and recovery (25℃ for 12hr) suggest that HSP70 was induced largely in hepatocytes at 46℃. The sterological methods was used to analysis the quantitative HSP70 expression.
出处
《河南科学》
1999年第1期58-62,共5页
Henan Science
基金
中法先进技术项目
