高系一四甘薯茎尖培养及快繁技术研究

Studies on stem tipculture and rapid propagation of sweet potato(Ipomoea batatas Lam)

通过筛选培养基，研究了高系一四甘薯茎尖培养及快速繁殖的方法．结果表明，ＭＳ＋６－ＢＡ０．１０～０．２５ｍｇ／Ｌ，ＩＡＡ０．０１ｍｇ／Ｌ或ＮＡＡ０．０５ｍｇ／Ｌ，ＧＡ０．０５ｍｇ／Ｌ是较好的茎尖诱导培养基，茎尖的萌动率为８０％以上，６５ｄ即可一次成苗．茎尖苗以茎切段进行增殖，经筛选，增殖培养基以ＭＳ＋６－ＢＡ０．０５～０．５０ｍｇ／Ｌ，ＮＡＡ０．００５～０．０５０ｍｇ／Ｌ，ＧＡ０．１ｍｇ／Ｌ为最好，２０～３０ｄ即可成苗，移栽成活率为１００％．

The methods of meristem tipculture and rapid propagation of sweet potato (Ipomoea batatas Lam) were studied by sifting culture medium.The MS medium suppl emented with 6 BA 0 10～0 25mg/L,IAA 0 1mg/L or NAA 0 05mg/L and GA 0 1mg/L is a medium suitable for stem tipculture.The germination rate of stem tips is over 80%.After 65 days,test tube plantlets are formed.The stem tips plantlets are propagated by culture of stem segments.By sifting,the stem segments grow well in MS medium supplemented with BA 0 05 0 50mg/L,NAA 0 005 0 050mg/L and GA 0 05mg/L.After 20～30 days,the test tube plantlets are formed.The survival rate of transplant is 100%.