抑癌基因bcl-2在玉米染色体中的荧光原位杂交定位

Physical Location of Tumor Suppressor Gene bcl 2 in Maize Chromosomes by FISH

以人的抑癌基因ｂｃｌ－２为探针，通过Ｓｏｕｔｈｅｒｎ杂交技术，发现ｂｃｌ－２在玉米和水稻的基因组中均具有同源序列；并运用荧光原位杂交技术，对玉米中的ｂｃｌ－２同源序列进行了定位．结果表明：ｂｃｌ－２在玉米第４号和第７号染色体上均检出了杂交信号，信号检出率分别为８．１６％和６．６３％．ｂｃｌ－２在第４号染色体长臂上的杂交信号与着丝粒的百分距离为５８．０７±４．９０，在第７号染色体长臂上的杂交信号与着丝粒的百分距离为８０．３４±３．１２．上述研究结果为研究植物细胞程序性死亡的可能调控机制提供了线索。

To analyze the presence of homologous sequences of human tumor supprossor gene bcl 2 in maize and rice, Southern hybridization was performed and positive signals were detected in these two species' genomes. FISH also was carried out for physical location of the bcl 2 homologous sequences in maize chromosomes. bcl 2 was hybridized onto the long arm of the fourth and the seventh chromosomes. The detection rates of FISH were 8.10% and 6.63%. The percentage distances from centromere to detection sites were 58.07±4.90 and 80.34±3.12 respectively. These results may provide important clues of the further research of the molecular mechanisms regulating the plant programmed cell death, and it may also benefit to further research on the relationship of programmed cell death between plants and animals.