卵巢切除所致肾虚对免疫性关节炎小鼠血清骨钙素和肿瘤坏死因子的影响

Effect of Kidney Deficiency Caused by Ovariectomy on Serum Osteocalcium Level and Tumor Necrosis Factor in Mice with Collagen Induced Arthritis

目的：探讨卵巢切除所致肾虚影响免疫性关节炎（痹证）中骨代谢的机制。方法：切除小鼠卵巢后，Ⅱ型胶原皮下注射以诱导关节炎（ＣＩＡ）；每周１次观察小鼠的关节肿胀程度；组织切片光镜下观察小鼠滑膜、关节软骨及骨的改变；放射免疫法检测小鼠血清雌二醇、骨钙素、肿瘤坏死因子的变化。结果：切除卵巢的小鼠，血中雌二醇水平明显下降，而血清骨钙素和肿瘤坏死因子的含量则显著增加；同时，滑膜组织炎症加重，软骨损伤及骨的破坏均更为严重。结论：卵巢切除所致肾虚可使ＣＩＡ的病变程度加重。

Objective: To explore the effect of ovariectomy caused Kidney Deficiency on the metabolism of bone in mice with collagen induced arthritis. Methods: The mice for experiment were immunizied with subcutaneous injection of type Ⅱ collagen to induce arthritis after ovariectomy. Severity of joint swelling, radioimmunoassay of serum estradiol (E 2),osteocalcin (OC) and tumor necrosis factor (TNF), and pathological changes of joint, including changes on synovia, articular cartilage and bone, were observed once weekly. Results: The E 2 level of ovariectomized mice dropped down obviously, while the contents of OC and TNF increased significantly. Severe pathological changes can be seen in synovial tissues, cartilage and bone. Conclusion: Kidney Deficiency caused by ovariectomy exacerbated the pathological changes of collagen induced arthritis in mice.