2007-2008年北京地区冬季肠道门诊腹泻患者人杯状病毒感染监测

Surveillance of human calicivirus infection in diarrheal patients visiting enteric clinics in Beijing in winter,2007-2008

目的调查北京地区冬季肠道门诊就诊腹泻患者人杯状病毒的感染情况。方法收集北京市2007年12月至2008年3月12家医院肠道门诊就诊腹泻患者271例,采集患者粪便标本,使用反转录-聚合酶链反应(RT-PCR)对粪便标本进行人杯状病毒RNA检测,对RT-PCR阳性标本的PCR产物进行克隆测序。结果检测271例粪便标本,共有87例阳性(87/271,32.10%)。选择其中21例PCR产物进行克隆测序,将获得的序列进行比对分析、构建系统发生树,结果表明,21株全部为诺如病毒,未见扎如病毒。其中GⅡ/4型16株(16/21,76.19%),GⅡ/6型1株(1/21,4.76%),GⅡ/7型1株(1/21,4.76%),GⅡ组未定型3株(3/21,14.29%)。结论北京地区冬季肠道门诊就诊的腹泻患者人杯状病毒感染较高,主要流行株为诺如病毒GⅡ/4型。

Objective To determine the prevalence of human calicivirus infection among diarrheal patients who visited enteric clinics in winter in Beijing.Methods From December 2007 to March 2008,271 diarrheal patients visiting enteric clinics were investigated in Beijing.Their fecal specimens were collected for the detection of human caliciviruses using the reverse transcription polymerase chain reaction（RT-PCR）,and positive PCR products were cloned and sequenced.Results A total of 87 positive cases were detected among the 271 patients,with the positive rate of（32.10%）,and 21 PCR products were randomly selected for sequencing.Compared with the nucleotide sequences of norovirus reference strains from GenBank,phylogenetic analysis showed that all of the 21 samples were norovirus other than sapovirus,including 16 isolate of norovirus GⅡ/4（16/21,76.19%）,1 isolate of norovirus GⅡ/6（1/21,（4.76%）,） 1 of norovirus GⅡ/7（1/21,4.76%） and the other 3 of unidentified type（3/21,14.29%）.Conclusion（The infection） rate of human calicivirus was relatively high among diarrheal patients visiting enteric clinics in winter in Beijing,and norovirus GII /4 was the predominant genotype.