细胞凋亡相关基因在食管上皮癌变中的表达及其意义

Expression and significance of regulatory genes of cell apoptosis in esophageal cancellation course

目的 探讨食管上皮癌变过程中多个相关的细胞凋亡调控因子的表达状况及其意义.方法 应用碘化丙啶染色和间接免疫荧光标记方法,采用流式细胞术对60例食管癌组织及相应的癌旁组织进行定量检测.结果 bcl-2、c-FLIP基因蛋白在食管癌中的表达皆显著高于癌旁组织,两者比较差异有统计学意义（P〈0.01） Fadd、Caspase-8和Caspase-3蛋白在食管癌中的表达皆显著低于癌旁组织,两者比较差异有统计学意义（P〈0.01）.基因相关性比较结果显示,Fadd与Caspase-8基因蛋白表达之间呈正相关关系（P〈0.01）,与正常黏膜组织和不典型增生组织相比,癌组织中DNA含量明显增高,异倍体细胞显著增加.结论 细胞凋亡的级联调控机制c-FLIP-Fadd-Caspase-8-Caspase-3-bcl-2在食管上皮癌变过程中起着重要作用.食管上皮癌变过程中,DNA含量及异倍体率增加.

Objective To explore the expression of cell apoptosis regulatory elements in esophageal canceration course. Methods 60 specimens of esophageal carcinoma and corresponding adjacent no cancerous tissue were detected quantitatively by propidium iodide（PI） stain and indirect immunofluorescence of fluorescein isothiocyanate（FITC） using flow cytometry（FCM） equipment. Results Expression of bcl-2 and c-FLIP in esophageal carcinoma was significantly higher, and Fadd, Caspase-8 and Caspase-3 were significantly lower than that in adjacent tissues （P 〈0.01）. There was positive correlation between Fadd and Caspase-8 gene （P 〈0.01 or P 〈0.05）. The expression of Fadd was positively correlated to Caspase-8. Comparing with normal epithelium and dysplasia tissue, DNA content and heteroploid cells were increased significantly in esophageal carcinoma. Conclusion During the esophageal canceration course, expressions of relevant cell apoptosis regulatory elements were abnormal. DNA content and heteroploid cells were increased significantly in esophageal carcinoma.