家蚕表达的重组hM-CSF对化疗损伤小鼠造血功能的恢复

Acceleration of hematopoietic recovery of chemotherapeutically lesioned mice by recombinant human macrophage colony stimulating factor expressed in silkworm

目的研究重组人巨噬细胞集落刺激因子（ｒｈＭ－ＣＳＦ）对化疗损伤小鼠骨髓造血功能恢复的作用。方法用环磷酰胺１００ｍｇ／ｋｇ连续３天给予小鼠腹腔注射，造成化疗损伤模型。继以不同剂量（０．１～２．０μｇ／天）的ｒｈＭ－ＣＳＦ连续７天给小鼠腹腔注射治疗。在化疗后第５，８，１１，１４天进行外周血白细胞总数及中性粒细胞、单核细胞和淋巴细胞的计数；并在化疗后第７天进行骨髓ＣＦＵ－ＧＭ的集落培养。数据均采用ｘ±ｓ，用ｔ检验比较差异显著性。结果１．外周血象变化的观察结果显示：化疗后第８天，注射０．５μｇ以上ｒｈＭ－ＣＳＦ治疗组的小鼠外周血白细胞总数非常显著高于对照组（Ｐ＜０．０１）；注射０．１μｇ以上的ｒｈＭ－ＣＳＦ单核细胞数和中性粒细胞数在化疗后第８天非常显著高于对照组（Ｐ＜０．０１）；其中以１．０μｇ剂量组的效果最佳。２．化疗后第７天骨髓ＧＭ－ＣＦＵ集落形成能力对照组集落数显著低于０．５μｇ以上剂量的ｒｈＭ－ＣＳＦ治疗组，加ｒｈＭ－ＣＳＦ作为刺激因子组和加ｒｈＭ－ＣＳＦ和Ｇ－ＣＳＦ作为刺激因子组的集落形成能力无明显区别。结论这种家蚕表达的ｒｈＭ－ＣＳＦ能促进化疗损伤小鼠造血功能的恢复，为其应用于临床治疗白细胞减少性疾病提?

Objective To investigate the effect of rhM CSF on hematopoietic recovery of chemotherapeutical lesions. Methods 100mg/kg CTX were ip injected into mice for 3 consecutive days to make the model of chemotherapeutical lesions. Mice were also ip injected with rhM CSF at doses of 0 1 2 0μg/day following the injection of CTX. Results 1 Observation of the peripheral hematological changes showed: The peripheral white blood cell count of treatment group(0 5 2 0 μg/day rhM CSF) was significantly higher than that of the control group at day 8 ( P <0 01). Monocyte and granulocyte counts of treatment group(0 1 2 0 μg/day rhM CSF) were significantly higher than those of the control group at day 8( P <0 01). Among which, 1 0 μg/day rhM CSF was most active. 2 On day 7, the bone marrow CFU GM yield of control group was significantly lower than that of treatment group, and no evident difference was found between the effect of rhM CSF and rhM CSF plus G CSF as stimulating factor. Conclusion rhM CSF could accelerate hematopoietic recovery in chemotherapeutically lesioned mice.