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有机相酶传感器的研究进展

Research progress of organic phase enzyme electrodes
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摘要 在生物传感器发展过程中,有机相酶电极因其是在有机介质中对物质进行检测,而受到关注。近年来,已经成为非水酶学具有重大应用价值的领域之一,并在不断创新的技术推动下稳步发展。介绍了有机溶剂对酶活性的影响,重点介绍了有机相酶电极的制备方法,并对未来的趋势进行展望。 In the development of biosensors,organic phase enzyme electrodes（OPEEs） have received considerable attention for the detection of substrates in organic media.In recent years,OPEEs was developed to an important and valuable field in nonaqueous enzymology,continuous innovation and improvements of technique allow OPEEs steady development.This paper introduces the effect of organic solvents on the activity of enzymes.Focus on the preparation methods of OPEEs and looks forward to the future development trend of OPEEs.
作者 陶莎 张惠 薛文通
机构地区 中国农业大学食品科学与营养工程学院
出处 《食品科技》 CAS 北大核心 2010年第7期29-32,共4页 Food Science and Technology
基金 国家自然科学基金资助项目(20876265) 质检公益性科研专项资助项目(200910034-02)
关键词 有机相酶传感器 酶活性 固定化方法 organic phase enzyme electrodes enzyme activity immobilization methods
分类号 Q814 [生物学—生物工程]
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参考文献21

  • 1Zaks A, Klibanov A M. Enzymatic catalysis in organic media at 100 degrees C[J]. Science,1984,224:1249-1251.
  • 2Fitzpatrick P A, Steinmetz A C U, Ringe D, et al. Enzyme crystal structure in a neat organic solvent[J]. Proceedings of the National Academy of Sciences of the USA,1993,90: 8653-8657.
  • 3Schmitke J L, Wescott C R, Klibanov A M. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents[J]. Journal of the American Chemical Society,1996,118:3360-3365.
  • 4Burke P A, Griffin R G, Klibanov A M. Solid-state NMR assessment of enzyme active center structure under nonaqueous condition[J]. Journal of Biological Chemistry, 1992,267:20057-20064.
  • 5Affleck R, Haynes C A, Clark D S. Solvent dielectric effects on protein dynamics[J]. Proceedings of the Nation- al Academy of Sciences,1992,89:5167-5170.
  • 6Schmitke J L, Wescott C R, Klibanov A M. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents[J]. Journal of the American Chemical Society,1996,118:3360-3365.
  • 7Wang Y, Guo Y Z, Zhu G Y, et al. Cryoimmobilized enzyme for biosensor construction[J]. Sensors and Actuators B,1996,31:193-196.
  • 8Burke P A, Griffin R G, Klibanov A M. Solid-State nuclear magnetic resonance investigation of solvent dependence of tyrosyl ring motion in an enzyme[J]. Biotechnology and Bioengineering,1993,42:87-94.
  • 9Cao S G, Feng Y, Liu Z B, et al. Lipase catalysis in organic solvents[J]. Applied Biochemistry and Biotechnology, 1992,32:7-13.
  • 10Mannino S, Cosio M S, et al. Determination of peroxide value in vegetable oils by an organic-phase enzyme electrode[J]. Analytical Letters,1994,27(2):299-308.

二级参考文献42

  • 1李业梅,刘慧宏,张志凌.肌红蛋白在海藻酸钠水凝胶中的电化学和电催化特性[J].化学研究与应用,2004,16(4):530-532. 被引量：2
  • 2李文江 等.高等学校化学学报,2001,9:1601-1601.
  • 3Brusova,Z.;Ferapontova,E.E.;Sakharov,I.Y.;Magner,E.;Gorton,L.Electroanalysis,2005,17:460
  • 4Ruzgas,T.;Csoregi,E.;Emneus,J.;Gorton,L.;Markovarga,G.Anal.Chim.Acta,1996,330:123
  • 5Liu,B.;Fang,Y.;Kong,J.;Deng,J.Anal.Chim.Acta,1999,386:31
  • 6Liu,H.;Lu,J.;Zhang,M.;Pang,D.Anal Sci.,2002,18:1339
  • 7Tatsuma,T.;Okawa,Y.;Watanabe,T.Anal.Chem.,1989,61:2352
  • 8Frew,J.F.;Hamer,M.A.;Hill,H.A.O.;Libor,S.I.J.Electroanal.Chem.,1986,201:1
  • 9Jia,J.;Wang,B.;Wu,A.;Cheng,G.;Li,Z.;Dong,S.Anal.Chem.,2002,74:2217
  • 10Luo,X.L.;Xu,J.J.;Zhang,Q.;Yang,G.J.;Chen,H.Y.Biosens.Bioelectron.,2005,21:190

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食品科技
2010年 第7期

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