D-101大孔树脂纯化多穗柯中根皮苷的工艺研究

Study on purification technology of phloridzin from Lithocarpus polystarch with D-101 macroporous resin

目的通过大孔树脂对多穗柯中的根皮苷吸附和解析研究,得到根皮苷的最佳纯化工艺。方法:采用超声波辅助提取多穗柯中的根皮苷,用HPLC法测定根皮苷的含量,测定7种大孔树脂(S-8、AB-8、D-101、NKA-9、NRR、X-5、D-3520)对根皮苷的吸附和解析,正交实验优化纯化工艺。结果:D-101型大孔树脂对多穗柯中的根皮苷具有较好的吸附和解吸效果,D-101对多穗柯中的根皮苷的最佳纯化工艺条件为:吸附速率为2.5mL/min,洗脱剂浓度为90%,pH值为8,解吸速率为4mL/min,通过极差分析发现各因素的影响次序为洗脱剂浓度>解吸速率>pH值>吸附流速。结论:大孔树脂D-101是纯化多穗柯中的根皮苷的最佳树脂,纯度达到95.6%,此工艺可行。

Objective obtain the best purification process of phloridzin from Lithocarpus polystarch according to the study on absorption and desorption of phloridzin with macroporous resin.Methods：Ultrasonics extraction was used to extract phloridzin.The content of phloridzin.from Lithocarpus polystarch was determined by HPLC.Absorption and desorption of phloridzin.with seven kind of macroporous resin（S-8,AB-8,D-101,NKA-9,NRR,X-5,D-3520）were determined.The optimum purification process was confimed by the orthogonal test.Results：The D-101 macroporous resin possessed higher absorption and desorption to phloridzin from Lithocarpus polystarch.The optimum conditions of purification are as follow：adsorption rate was 2.5 mL/min,eluant concentration was 90%,pH was 8,desorption rate was 2 mL/min.The effects of factors on yield decrease progressively in the following order：eluant concentration desorption rate pH adsorption rate.Conclusion：The best resin of purification of phloridzin From Lithocarpus polystarch is macroporous resin D101,purity reached 95.6%,this process is feasible.