原花青素对慢性应激大鼠抑郁样行为和肾上腺结构的影响

The effects of proanthocyanidins on antidepressant-like behaviors and the adrenal gland in chronic stress rats

目的 研究原花青素对慢性应激抑郁模型大鼠行为活动和肾上腺结构的影响.方法 采用慢性不可预见性温和应激模型,连续造模给药21 d后,测定大鼠开野活动,强迫游泳不动时间和糖水消耗量;测定应激大鼠肾上腺体质量比率和皮质厚度,HE染色观察应激大鼠肾上腺结构的改变.结果 原花青素（25,50,100 mg·kg^-1）组[（49±4.5）次,（52.6.±3.7）次,（54.1±1.8）次]的开野活动相比慢性应激模型组[（39.6 ±3.4）次]显著增加（均P〈0.001）;原花青素（25,50,100 mg·kg^-1）[（83.7 ±8.8）s,（75.8±5.9）s,（67.2 ±6.5）s]相比慢性应激组[（103.5 ±10.2）s]明显缩短大鼠的不动时间（均P〈0.01）;原花青素（25,50,100 mg·kg^-1）[（8.1 ±3.3）ml,（8.5 ±4.1）ml,（9.2 ±2.6）m1]相比慢性应激模型组[（5.8±2.5）ml]明显增加大鼠糖水摄入量（P〈0.05）;原花青素（25,50,100 mg·kg^-1）相比慢性应激模型能明显减小肾上腺体质量比率（P〈0.05）,减轻应激大鼠的肾上腺皮质厚度,差异有显著性（P〈0.05,P〈0.01）.结论 原花青素能明显改善慢性不可预见性应激模型大鼠的抑郁样行为活动,对应激所致肾上腺的结构改变也有保护作用.

Objective To investigate the effects of proanthocyanidins on depressant-like behaviors and the structure of adrenal gland in chronic unpredictable mild stress （CUMS） rats. Methods Rats were randomly divided into 6 groups：control group, stressed group （CUMS ＋ vehicle）, three treatment groups （CUMS ＋ proanthocyanidins 25,50,100 mg·kg^-1,respectively） ,and imipramine group （CUMS ＋ imipramine 10 mg·kg^-1）. Used the CUMS model in rats to investigate the effects of chronic oral administration （21 days） of proanthocyanidins and imipramine （ip） on the open-field;and forced swimming and sucrose consumption tests and the ratio of adrenal gland/body weight,and its thickness were examined by HE stain. Results Compared with control group, rats subjected to CUMS exhibited increased ratio of adrenal gland /body weight （ P 〈 0. 01）, less sucrose consumption（ P〈0.01） and inhibited in the open-field test（ P〈0.01） as well as more despair time in the forced swimming test（ P〈0.01）. While compared with stressed group,treatments with proanthocyanidins （25,50,100 mg·kg^-1, po ,21 days） could significantly improve the activities in open-field test （（39.6±3.4） vs （49±4.5）, （52.6±3.7）,（54.1±1.8） ;all P〈0.01） and sucrose consumption（ （5.8±2.5）ml vs （8.1±3.3）ml,（8.5±4.1） ml, （9.2±2.6） ml; P 〈 0.05, P 〈 0.05, P 〈 0.01 respectively）; Meanwhile, it could reduce the duration time in forced swimming test significantly（ （103.5±10.2）s vs （83.7±8.8）s,（75.8±5.9）s,（67.2±6.5）s; all P〈0.01） as well as thickness of the adrenal gland（P〈0.05, P〈0.01）.Conclusions This study suggests that the proanthocyanidins （25,50,100 mg·kg^-1） has an antidepressant-like effects in CUMS rats. The antidepressant actions of proanthocyanidins, in some degrees, may be related with the regulation of the adrenal gland＇s structure.