近视Ⅲ号方对气虚大鼠环核苷酸的影响

Experimental study on effect of jinshifangⅢ on cyclonucleotide in the rats with deficiency of qi

目的：探讨近视Ⅲ号方对气虚血滞高度近视黄斑病变的作用机理。方法：选用１２月龄大鼠，随机分成４组。模型组用大剂量生大黄煎剂灌胃，治疗组分别用小剂量及大剂量近视Ⅲ号方加生大黄煎剂灌胃，空白对照组灌生理盐水，２０天后分别取大鼠血浆和眼球视网膜，测定其ｃＡＭＰ、ｃＧＭＰ含量。结果：模型组ｃＡＭＰ、ｃＡＭＰ／ｃＧＭＰ均低于空白对照组（Ｐ＜０．０５），而治疗组之大剂量组与空白对照组之间则无显著性差异（Ｐ＞０．０５）。结论：近视Ⅲ号方能促使气虚大鼠ｃＡＭＰ、ｃＡＭＰ／ｃＧＭＰ升高。

OBJECTIVE: To study the mechanism of jinshifang Ⅲ on maculopathy in high myopia with deficiency of qi and blood stasis. METHODS: The 12 months old rats were divided into 4 groups randomly. The rats of model group were administered with raw rhubarb decoction, while the rats of treated groups were administered respectively with large dose and small dose jinshifang Ⅲ decoction when raw rhubarb decoction also was administered, and the rats of control group were administered with normal saline. After these trials had been performed for 20 days, the levels of cAMP and cGMP were determined in rat blood plasma and retina. RESULTS: The levels of cAMP and cAMP/cGMP in model group were lower than that of control group ( P <0 05 ), while there was not significant difference between the treated group with large dose and normal control group ( P >0 05). CONCLUSIONS: Jinshifang Ⅲ can raise the levels of cAMP and cAMP/cGMP in the rats with deficiency of qi.