青稞生长期间叶片中大麦黄苷与皂草黄苷的积累及品种间差异

Accumulation of Lutonarin and Saponarin in Barley Leaves during Growth and Differences in Their Contents among Different Varieties

采用高效液相色谱法,测定青稞叶片中两种黄酮大麦黄苷与皂草黄苷在生长期间的积累变化以及20个青稞品种叶片中大麦黄苷与皂草黄苷的含量差异。结果表明:在生长期内,大麦黄苷与皂草黄苷的积累变化趋势基本一致,均呈先增高后降低的趋势,但皂草黄苷的含量明显高于大麦黄苷,最佳的青稞叶采集期为三叶期至三叶一心期之间;在20种青稞品种中,白康青叶片中大麦黄苷含量最高,昆仑8号叶片中的皂草黄苷含量最高,康青3号叶片中的大麦黄苷和皂草黄苷含量最低,而两种黄酮总量也以昆仑8号最多,含量为1.69%,其次为白康青和昆仑1号。因此,以青稞黄酮含量为指标选用的品种是昆仑8号、白康青和昆仑1号。

The accumulation of lutonarin and saponarin during different growth and the differences in their contents among the leaves of different barley varieties were determined by HPLC. Results indicated that the content of saponarin was higher than that of lutonarin throughout the whole growth period and both of them displayed a similar change trend, i.e., an initial increase followed by a decrease. The optimal time for harvesting barley leaves was between the stage with three leaves and the stage with three leaves and one heart. Of 20 barely varieties, Baikangqing and Kunlun No.8 exhibited the highest contents of lutonarin and saponarin, respectively; however, the lowest contents of lutonarin and saponarin were both determined in Kangqing NO.3. In addition, Kunlun No.8 exhibited the highest total contents （1.69%） of lutonarin and saponarin, followed by Baikangqing and Kunlun No.1. Therefore, Kunlun NO.8, Baikangqing and Kunlun NO.1 are the selected barley varieties for the purpose of exploiting flavonoid resources.