乳铁蛋白铬对实验性糖尿病小鼠血糖水平的影响

Hypoglycemic Effect of Lactoferrin-chromium Complex in Experimental Diabetic Mice

目的:研究乳铁蛋白铬对四氧嘧啶诱发的糖尿病小鼠血糖水平的影响。方法:以糖尿病对照组(生理盐水,10mL/(kgbw·d))为阴性对照组,盐酸二甲双胍片组(250mg/(kgbw·d))为阳性对照组,观察灌胃乳铁蛋白(1.4、7、14mg/(kgbw·d))及乳铁蛋白铬络合物(1.4、7、14mg/(kgbw·d);按铬含量计为1.67、8.35、16.7μg/(kgbw·d))不同时间的降糖作用。结果:灌胃7d时,与糖尿病对照组相比,乳铁蛋白铬络合物(14mg/(kgbw·d))对糖尿病小鼠有显著降低血糖(P<0.05)及改善葡萄糖耐量作用(P<0.01);灌胃14d时,与糖尿病对照组相比,乳铁蛋白铬络合物(7、14mg/(kgbw·d))对糖尿病小鼠有极显著降低血糖及改善葡萄糖耐量作用(P<0.01);灌胃7、14d时,与糖尿病对照组相比,乳铁蛋白无显著降低血糖及改善葡萄糖耐量作用(P>0.05)。结论:在实验剂量范围内,随着乳铁蛋白铬络合物剂量的增加,和食用时间的延长,对实验性糖尿病小鼠的降血糖效果和改善葡萄糖耐量作用会越明显,而乳铁蛋白则无显著降糖功效。

In order to explore the hypoglycemic effect of lactoferrin-chromium in mice with experimental diabetes mellitus induced by alloxan, the diabetic animals were randomly divided into 3 lactoferrin groups administered lactoferrin at varying dosages （i.e., 1.4, 7 mg/（kg bw·d） and 14 mg/（kg bw·d）, 3 lactoferrin-chromium complex groups administrated lactoferrin- hromium complex at the same dosages as those of lactoferrin （1.67, 8.35 μg/（kg bw·d） and 16.7μg/（kg bw·d）, calculated on the basis of chromium amount）, negative control group administered saline at 10 mL/（kg bw·d）, positive control group administered diformin at 250 mg/（kg bw·d）. All the animals were administered once a day for 14 consecutive days. The results indicated that the high-dosage lactoferrin-chromium complex groups after 7 days of administration displayed a significantly lower blood glucose level （P 0.05） and a significant higher glucose tolerance （P 0.01） when compared with the negative control group. After 14 days, a significantly lower blood glucose level and a significantly higher glucose tolerance （P 0.01） were observed in the medium- and high-dosage lactoferrin-chromium complex groups. In contrast, after 7 and 14 days, lactoferrin failed to cause significant changes in blood glucose level and glucose tolerance （P 0.05） when compared with diabetic control group. Therefore, lactoferrin-chromium complex has significant hypoglycemic and glucose tolerance improving effects in diabetic mice in dose-dependent and time-dependent manners.