菜籽饼粕多糖对D-半乳糖衰老小鼠的抗氧化及免疫调节作用

Antioxidant and Immunomodulatory Effects of Polysaccharide from Rapeseed Meal in D-galactose-induced Aged Mice

研究菜籽饼粕多糖(polysaccharide from rapeseed meal,PRM)对D-半乳糖(D-galactose,D-gal)亚急性衰老模型小鼠的抗氧化及免疫调节作用。采用小鼠D-半乳糖人工衰老模型,从氧自由基代谢和免疫调节方面进行研究。结果表明,PRM(400、200mg/kgbw)可明显降低D-gal衰老小鼠肝肾组织中过高的MDA含量,上调其过低的抗氧化酶超氧化物歧化酶(SOD)活性。PRM还可明显提高D-gal衰老小鼠的胸腺指数,恢复其低下的脾淋巴细胞ConA増殖反应与白介素2(IL-2)产生;降低D-gal衰老小鼠升高的巨噬细胞增殖反应、白介素1(IL-1)和NO产生,说明PRM具有提高机体保护性免疫反应、抑制破坏性免疫反应的作用。因此,PRM对D-gal衰老模型小鼠具有抗氧化和免疫调节作用。

Subcutaneous injection of D-galactose was used to artificially induce aging in mice and the aged mice were randomly divided into 4 groups to investigate the in vivo antioxidant and immunomodulatory effects of polysaccharide from rapeseed meal （PRM） by evaluating oxygen free radical metabolism and immunomodulatory activities. The results showed that PRM at 400 and 200 mg/kg bw obviously reduced the higher MDA contents and unregulated the lower SOD activities in liver and kidney of D-galactose-induced aged mice. Also, PRM not only increased the thymus index as well as lower ConA proliferation response of spleen lymphocytes and IL-2 production, but also reduced the proliferation response of macrophages and the generation of IL-1 and NO in D-galactose-induced aged mice. These investigations demonstrate that PRM is able to improves protective immune response and inhibit destructive immune response. Therefore, PRM has remarkable antioxidant and immunomodulatory effects in D-galactose-induced aged mice.