低氧对人骨髓间充质干细胞向胆碱能神经元分化的影响(英文)

Effect of hypoxia on the differentiation of human bone marrow mesenchymal stem cells into cholinergic neurons

【目的】观察低氧对体外培养的人骨髓间充质干细胞(hBMSCs)向胆碱能神经元分化的影响。【方法】将对照组和常氧分化组hBMSCs置于常氧(20%O2)环境中培养,低氧分化组细胞置于低氧(3%O2)环境中。采用免疫细胞化学法和高效液相色谱—电化学法,分别检测胆碱能神经元的数量和诱导分化后乙酰胆碱(ACh)的含量。【结果】对照组未见胆碱乙酰化酶(ChAT)阳性神经元,低氧分化组ChAT阳性神经元数量明显增多,约为常氧分化组的4倍(P<0.01),并且该组诱导分化后的细胞合成的ACh含量也高于常氧分化组(P<0.05)。【结论】低氧可促进hBMSCs向胆碱能神经元方向分化,这为临床应用hBMSCs治疗阿尔茨海默病提供了新的思路。

[Objective] To investigate the effect of hypoxia on the differentiation of human bone marrow mesenchymal stem cells （hBMSCs） into cholinergic neurons in vitro. [Methods] hBMSCs in the control group and the normoxic differentiation group were cultured under normoxic condition （20% O2）, while cells in the hypoxic differentiation group were in hypoxic environment （3% O2）. The number of differentiated cells was counted by immunocytochemical method and the content of acetylcholine （ACh） was measured by the electro-chemical detection of high-performance liquid chromatography （HPLC-ECD）. [Results] Choline acetyhransferase （ChAT）-positive cells were not observed in the control group. ChAT-positive cells differentiated from the induced hBMSCs under hypoxic condition increased about 3 times as compared with that under normoxic condition （P〈0.01）. Furthermore, the content of ACh of the differentiated cells in hypoxia group was significantly higher than that of normoxic group （P〈0.05）. [Conclusions] Hypoxia improves the differentiation of hBMSCs into cholincrgic neurons, which provides a new idea for clinical treatment of Alzheimer＇ s disease with hBMSCs.