AP-1、Integrin α_1的表达和肺癌转移的相关性

Expression of AP-1,integrin α_1 and the relationship with the metastasis of of lung cancer

【目的】研究AP-1、integrin α1、Ⅷ因子在人肺癌中的表达及其与肺癌临床病理特征的关系。【方法】通过免疫组织化学方法检测101例肺癌组织及7例正常肺组织中AP-1、integrin α1、Ⅷ因子的表达情况,利用CMIAS2000型多功能真彩病理图象分析系统测量计算各病例中c-jun、c-fos、integrin α1蛋白阳性细胞的平均光密度(AOD)和积分光密度(IOD)。并以Ⅷ因子抗体阳性血管数计算微血管密度(MVD)。【结果】C-jun、c-fos在肺癌组织中表达的阳性率及其共表达率均显著高于正常肺组织;二者在NSCLC的表达显著强于SCLC;不同组织学类型肺癌中二者表达具有显著性差异,腺癌中表达最强,小细胞肺癌中表达最弱;c-jun、c-fos与肺癌分级、合并分期、淋巴结转移均呈显著正相关。Integrin α1与肺癌淋巴结转移密切相关,与合并分期显著正相关;integrin α1与AP-1之间均存在显著正相关关系;Integrin α1与MVD间存在显著正相关性。【结论】AP-1信号传导通路通过上调integrin α1的表达,改变细胞粘附特性、促进新生血管建立,从而加速了肺癌的浸润转移。

[Objective] To explore the expression of AP-1（c-jun/c-fos）,integrinα1 and factorⅧ in lung cancer specimens and to find out the correlation between them and the clinical feature of lung cancer.[Methods] The expression of c-jun、c-fos、integrinα1 and factorⅧwere detected with S-P immunohistochemical method in 101 cases of paraffin-embedded sections of lung cancer tissues and 7 normal lung tissues as control.[Results] The expression levels of c-jun,c-fos and their common expression levels in the 101 cases of lung cancer tissues were significantly higher than that of the 7 cases control（P0.001）.The expression of c-jun and c-fos is significantly higher in NSCLC than that in SCLC and had a positive correlation with grades,combined staging and lymphatic metastasis of lung cancer.Integrinα1 and a osculate correlation with lymphatic metastasis and combined staging of lung cancer.There were significantly positive correlation between AP-1 and integrinα1,integrinα1 and MVD.[Conclusions] Through up-regulating the expression of integrinα1,changing the cell adherence character,promoting the neovascularization,AP-1 accelerates the invasion and metastasis of lung cancer.