苋菜红蓄积毒性试验研究

Cumulative toxicity of amaranth

目的观察苋菜红对实验大鼠的蓄积毒性,确定可能的毒副反应靶器官,为深入研究提供参考。方法 Wistar大鼠随机分2组,分别为苋菜红组和对照组。苋菜红组采用剂量递增方法给药,即于第1～4天ig给予1/10LD50(LD50按10g/kg计),以后每4d按前一剂量0.5倍递增1次,累计自实验开始至出现50%动物死亡期间的总剂量[∑LD50(n)];对照组给以蒸馏水。观察实验期间大鼠的一般毒性症状、体质量变化、死亡情况,进行生化检测,尸检肉眼观察器官病变。结果实验期间动物的精神、外观均无异常。体质量、血液学、血液生化学等各项指标均无明显异常。结论苋菜红对动物无明显蓄积毒性。

Objective To evaluate the toxic reactions of amaranth and determine the possible target organs so as to provide certain reference for further research. Methods Rats were divided randomly into two groups, amaranth group and control group. In amaranth group, rats were given orally different doses of amaranth increased continuously. In the first 1-4 days, the dose was one tenth of the LD50 dose （LD50 by 10 g/kg）. Then the dose increased by 1.5 times once every 4 days until the death of 50% rats. The cumulative total dose （∑LD50 （n）） was obtained. Rats in control group were given distilled water. During the experiment, general toxic condition, body weight and death were observed, and biochemical tests were conducted. In addition, postmortem findings were also observed with naked eyes. Results During the experiment, there were no markedly abnormal changes in animal energy, appearance, body weight, hematological and blood biochemical parameters. Conclusion Amaranth does not show significant accumulative toxicity to animals.