运用实时荧光定量PCR技术检测424例患者HPV结果分析

Analysis of 424 Cases HPV Detection with Quantitative Real-Time PCR

目的探讨采用实时荧光定量PCR反应(qPCR)检测人乳头瘤病毒(HPV)的感染情况及检测HPV分型的临床意义。方法用实时荧光定量PCR(qPCR)技术检测424例门诊患者阴道分泌物中HPV6/11和HPV8个基因型(HPV16、18、31、33、45、52、56、58型)的病毒拷贝数。结果共检出HPV6/11阳性者99例,阳性率为23%,其中<20岁年龄组阳性率最高,达到50%;共检出HPV8个基因型阳性者103例,阳性率为24%,其中>50岁年龄组阳性率最高,达到42%。结论 qPCR检测技术具有灵敏度高、特异性强及操作快捷等特点,可为HPV感染患者的临床治疗及预后观察提供可靠依据,具有较高的临床应用价值。

Objective To detect the human papillomavirus（HPV） genotyping with quantitative real-time PCR（qPCR） and to explore the clinical significance.Methods The quantitative real-time PCR（qPCR） was used to detect HPV 6/11and HPV 8（HPV16、18、31、33、45、52、56、58）DNA in 424 secretion samples.Results 99 HPV6/11 positive samples were detected with a positive rate of 23%;103 HPV8 positive samples were detected with a positive rate of 24%.Conclusion With high sensitivity,rapidity and accuracy,qPCR is suitable for clinical application.Also it can provide reliable information of treatment and prognosis for patients.