银屑病患者外周血单个核细胞 (PBMC) Sirt1,mRNA的表达及其意义

Investigation on Sirt1 in Peripheral Blood Mononuclear Cell (PBMC) and Relationship with Pathogenesis of Psoriasis

目的:从细胞凋亡角度探讨Sirt1在银屑病发病中的作用。方法:采用密度梯度离心法分离30例银属病患者和10例健康对照者外周血单个核细胞,实时定量RT-PCR(real-time RT-PCR)法检测PBMC细胞组蛋白去乙酰化酶Sirtl mRNA表达水平。结果:银屑病患者PBMC细胞Sirtl mRNA表达水平较正常对照组明显增加。结论:推测银屑病的角质形成细胞增殖可能与凋亡抑制因子Sirtl关系密切。

Objective：To investigated the role of Sirtl in the pathogenesis of psoriasis. Methods：30 psoriasis patients and 10 healthy controls were recruited. PBMC were isolated by Ficoll-Hypaque density gradient centrifugation, the mRNA levels of Sirtl genes were measured by real-time quantitative polymerase chain reaction. Results：Sirt1 mRNA levels were significantly increased in psoriasis PBMC compared with healthy controls （P〈0.001）. Conclusion：Sirt1 can promote the proliferation of keratinocytes and maintain the hyperproliferative state of psoriatic epidermis,which may partly by apoptosis interference.