藏药加哇中黄酮类成分和阿魏酸的定量分析

Determination of Three Flavonoids and Ferulic Acid in Pleurospermum hookeri C.B.Clarke var.thomsonii C.B.Clarke by HPLC

目的：建立藏药加哇中甘草查耳酮甲、阿魏酸、异甘草素和川陈皮素的含量测定方法。方法：ＯＤＳ柱，流动相甲醇１％醋酸（７３∶２７，２８∶７２，５９∶４１）和甲醇水（６５∶３５），检测波长３４５，３２０，３４５，３３２ｎｍ。结果：甘草查耳酮甲、阿魏酸、异甘草素和川陈皮素含量分别为０．７２８，０．１２４，０．０８３０，０．０２３７ｍｇ／ｇ，平均回收率分别为９９．４５％，９８．１３％，９７．８１％，９６．５８％。结论：为藏药加哇的质量评价。

Objective:To establish a reversed phase HPLC method for separation and determination of licochalcone A,ferulic acid,isoliquiritigenin and nobiletin in Pleurospermum hookeri var.thomsonii.Method:Using ODS column with methanol water(containing 1% acetic acid)(73∶27,28∶72,59∶41) and methanol water(65∶35) as the mobile phase,the flow rate was 1ml/min,the column temperature was 35℃ and UV detection at 345,320,345,332nm,different extraction methods for the sample were compared.Result:The average recoveries were 99.45%,98.13%,97.81% and 96.58%,the relative standard deviations were 0.83%,0.99%,0.73% and 1.55%,and the contents were 0.728,0.124,0.0830 and 0.0237mg/g.Conclusion:This study provides a scientific basis for quality evaluation,quality control and resources development and utilization of P.hookeri var.thomsonii.