CDC25B和Cyclin B1在胃腺癌中的表达及其临床意义

Expression of CDC25B and cyclin B1 in human gastric adenocarcinoma and its clinical significance

目的:探讨CDC25B和Cyclin B1在判断胃腺癌的病情及预后中的价值。方法:选取胃腺癌病例60例和正常胃组织15例作为本课题的研究对象。采用免疫组化法检测胃腺癌组织和正常胃组织中CDC25B和Cyclin B1的表达情况,结合临床病理和随访资料,运用统计学方法,回顾性分析CDC25B和Cyclin B1的表达和各项临床病理指标及预后之间的关系。结果:在胃腺癌中和正常胃组织中CDC25B的阳性表达率分别为46.7%和6.7%,而Cyclin B1的阳性表达率分别为70.0%和13.3%,差异有显著性。胃腺癌中CDC25B和Cyclin B1的阳性表达与浸润深度、TNM分期及淋巴结转移相关。生存率的单因素分析显示,浸润深度、TNM分期及淋巴结转移、CDC25B的表达和Cyclin B1的表达与胃腺癌的预后相关。生存率的多因素分析显示CDC25B和Cyclin B1的表达提示预后不良。未发现CDC25B和Cyclin B1在胃腺癌中的表达存在正性线性相关关系。结论:CDC25B和Cyclin B1的阳性表达提示胃腺癌预后不良。

Objective：To detect the expression of CDC25B and Cyclin B1 in human gastric adenocarcinoma,then to evaluate the clinical significance and correlation between the expression and prognosis of patients in gastric adenocarcinoma.Methods：Sixty patients of gastric adenocarcinoma and 15 control specimens from the normal gastric tissue were selected as objects.The retrospective study was conducted at the same time.The expressions of CDC25B and Cyclin B1 were analyzed in 60 gastric adenocarcinoma cases and 15 control specimens by immunohistochemistry assay.Results：The positive expression rates of CDC25B in gastric adenocarcinoma and in normal gastric tissue were 46.7% and 6.7%,while the positive expression rates of Cyclin B1 in gastric adenocarcinoma and in normal lung tissue were 70.0% and 13.3%.Differences were significant.The positive expression of CDC25B and Cyclin B1 in gastric adenocarcinoma was correlated with depth of tumor invasion,TNM stage or pathologic lymph node status.Univariate analysis demonstrated that the survival rate of 60 gastric adenocarcinoma cases was significantly affected by depth of tumor invasion,TNM stagep,athologic lymph node status and expression of CDC25B or Cyclin B1.Multivariate analysis（COX proportional hazards modeling） revealed that combination of expression of CDC25B and Cyclin B1 was a valuable marker to indicate a poor prognosis for patients with gastric adenocarcinoma.No positive linear correlation was noted between the expression of CDC25B and Cyclin B1 in gastric adenocarcinoma.Conclusion：Combination of expression of CDC25B and Cyclin B1 is a valuable marker to indicate a poor prognosis for patients with gastric adenocarcinoma.