摘要
目的:研究血管内皮生长因子(VEGF)和第10号染色体缺失的磷酸酶和张力蛋白同源物基因(PTEN)在非小细胞肺癌(NSCLC)中的表达及其与预后的关系。方法:采用免疫组织化学染色法检测60例NSCLC组织和10例正常肺组织中VEGF和PTEN的表达,结合临床病理和随访资料进行生存分析。结果:在NSCLC和正常肺组织中VEGF的阳性表达率分别为68.3%和10.0%(P<0.05),而PTEN的阴性(缺失)表达率分别为43.3%和0(P<0.05)。NSCLC中VEGF的阳性表达与p-TNM分期及淋巴结是否转移相关(P<0.05),而PTEN的阴性表达与分化程度、p-TNM分期及淋巴结是否转移相关(P<0.05)。VEGF和PTEN在NSCLC中的表达呈负相关(P=0.017,r=0.306)。Kaplan-Meier单因素分析结果表明,分化程度、p-TNM分期及淋巴结是否转移、VEGF的表达和PTEN的表达与NSCLC的预后相关(P<0.05)。COX多因素分析结果显示p-TNM分期和PTEN表达是影响NSCLC预后的独立危险因素(P<0.05)。结论:VEGF在NSCLC中过度表达,而PTEN在NSCLC中表达降低,两者在NSCLC的发生、发展中可能起相反的作用,影响NSCLC的预后。
Objective:To study the expression of VEGF and PTEN in NSCLC and their correlation with prognosis.Methods:The expression of VEGF and PTEN was measured in 60 NSCLC and 10 control specimens with immunohistochemistry assay,and its relation to the prognosis.Results:The positive expression rates of VEGF in NSCLC and in normal lung tissue were 68.3% and 10.0%(P〈0.05),while the negative expression rates of PTEN in NSCLC and in normal lung tissue were 43.3% and 0.00%(P〈0.05).The positive expression of VEGF in NSCLC was correlated with p-TNM stage or lymphatic metastasis(P〈0.05).The negative expression of PTEN in NSCLC was correlated with grading,p-TNM stage or lymphatic metastasis(P〈0.05).The expression of VEGF was negatively correlated with the expression of PTEN in NSCLC(P=0.017,r =0.306).Kaplan-Meier analysis showed that the survival rate of 60 NSCLC was significantly affected by grading,p-TNM stage,lymphatic metastasis,expression of VEGF or PTEN(P〈0.05).Multivariate COX model analysis revealed that p-TNM stage and expression of PTEN were independent prognostic factors of NSCLC(P〈0.05).Conclusion:The overexpression of VEGF in NSCLC may contribute to invasion and metastasis,while PTEN may be a beneficial prognosis factor in patients with NSCLC.
出处
《温州医学院学报》
CAS
2010年第4期381-385,共5页
Journal of Wenzhou Medical College