VEGF和PTEN在非小细胞肺癌中的表达

Expression of VEGF and PTEN in NSCLC

目的:研究血管内皮生长因子(VEGF)和第10号染色体缺失的磷酸酶和张力蛋白同源物基因(PTEN)在非小细胞肺癌(NSCLC)中的表达及其与预后的关系。方法:采用免疫组织化学染色法检测60例NSCLC组织和10例正常肺组织中VEGF和PTEN的表达,结合临床病理和随访资料进行生存分析。结果:在NSCLC和正常肺组织中VEGF的阳性表达率分别为68.3%和10.0%(P<0.05),而PTEN的阴性(缺失)表达率分别为43.3%和0(P<0.05)。NSCLC中VEGF的阳性表达与p-TNM分期及淋巴结是否转移相关(P<0.05),而PTEN的阴性表达与分化程度、p-TNM分期及淋巴结是否转移相关(P<0.05)。VEGF和PTEN在NSCLC中的表达呈负相关(P=0.017,r=0.306)。Kaplan-Meier单因素分析结果表明,分化程度、p-TNM分期及淋巴结是否转移、VEGF的表达和PTEN的表达与NSCLC的预后相关(P<0.05)。COX多因素分析结果显示p-TNM分期和PTEN表达是影响NSCLC预后的独立危险因素(P<0.05)。结论:VEGF在NSCLC中过度表达,而PTEN在NSCLC中表达降低,两者在NSCLC的发生、发展中可能起相反的作用,影响NSCLC的预后。

Objective：To study the expression of VEGF and PTEN in NSCLC and their correlation with prognosis.Methods：The expression of VEGF and PTEN was measured in 60 NSCLC and 10 control specimens with immunohistochemistry assay,and its relation to the prognosis.Results：The positive expression rates of VEGF in NSCLC and in normal lung tissue were 68.3% and 10.0%（P〈0.05）,while the negative expression rates of PTEN in NSCLC and in normal lung tissue were 43.3% and 0.00%（P〈0.05）.The positive expression of VEGF in NSCLC was correlated with p-TNM stage or lymphatic metastasis（P〈0.05）.The negative expression of PTEN in NSCLC was correlated with grading,p-TNM stage or lymphatic metastasis（P〈0.05）.The expression of VEGF was negatively correlated with the expression of PTEN in NSCLC（P=0.017,r =0.306）.Kaplan-Meier analysis showed that the survival rate of 60 NSCLC was significantly affected by grading,p-TNM stage,lymphatic metastasis,expression of VEGF or PTEN（P〈0.05）.Multivariate COX model analysis revealed that p-TNM stage and expression of PTEN were independent prognostic factors of NSCLC（P〈0.05）.Conclusion：The overexpression of VEGF in NSCLC may contribute to invasion and metastasis,while PTEN may be a beneficial prognosis factor in patients with NSCLC.