摘要
旨在研究猪呼吸道IgA和IgG分泌细胞的分布。取健康50日龄猪的软腭扁桃体、咽扁桃体、气管和肺组织,应用免疫组织化学技术研究猪呼吸道中IgA和IgG分泌细胞的变化。结果显示,在软腭扁桃体中,IgA和IgG分泌细胞主要分布在隐窝上皮下;在咽扁桃体中,IgA和IgG分泌细胞主要集中分布在黏膜上皮下;在气管中,IgA和IgG分泌细胞主要分布在黏膜上皮下固有层和气管腺周围,在气管上皮之间也有少量IgA分泌细胞分布;肺中的IgA和IgG分泌细胞主要集中分布在肺泡隔和各级支气管的黏膜上皮下。另外,我们观察到肺中的2种分泌细胞着色明显比气管中淡。对2种分泌细胞进行统计学分析发现,IgA分泌细胞在气管中分布最多,其次是咽扁桃体、软腭扁桃体,肺中最少;而IgG分泌细胞则在咽扁桃体中最多,其次是气管、肺,软腭扁桃体中最少。结果表明,猪呼吸道含有较多的抗体分泌细胞,但不同部位不同的抗体分泌细胞分布不尽相同,该结果可为猪呼吸道黏膜免疫及相关疫病发生机理的研究提供理论依据。
This experiment was conducted to study the localization of IgA and IgG in porcine respiratory tract.The tissue samples of soft palatine tonsil,pharyngeal tonsil,trachea and lung were collected from pigs(50 days) and studied by immunohistochemistry methods for detection of the IgA and IgG secreting cells in porcine respiratory tract.The results showed that the IgA and IgG secreting cells in soft palatine tonsil were distributed mainly underneath the crypt epithelium.In pharyngeal tonsil,the secreting cells were predominantly found underneath the mucosal epithelium.In trachea,the secreting cells were observed just beneath the mucosal epithelium.Some were also found around the tracheal glands.A few IgA secreting cells were found between the tracheal epithelium.In lung,the secreting cells were located in alveolar septum,and underneath bronchus and bronchiole epithelium.In addition,the secreting cells in lung stained much lighter than that in trachea.Statistics analysis showed that the number of IgA secreting cells was the most in trachea,then the pharyngeal tonsil,the soft palatine tonsil,the lung had the least number of IgA secreting cells.However,the number of IgG secreting cells were the most in pharyngeal tonsil,then the trachea,the lung,the soft palatine tonsil had the least number of IgG secreting cells.Such a wide distribution of the IgA and IgG secreting cells in porcine respiratory tract provide a foundation and knowledge to respiratory mucosa immunity and pathogenesis study on the pig′s disease.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第7期873-877,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家公益性农业行业科研专项(200803020)