中国小反刍兽疫病毒分离株China/Tib/07全基因组序列测定与分析

Genome Sequencing and Analysis of a Peste Des Petits Ruminants Virus Isolate,China/Tib/07

小反刍兽疫病毒属于副黏病毒科麻疹病毒属成员。本研究对我国首次分离的小反刍兽疫病毒株China/Ti-bet/07进行了全基因组序列测定及分子生物学特征分析。根据GenBank公布的小反刍兽疫病毒基因组序列设计引物通过RT-PCR扩增病毒基因组内部序列,通过3′和5′-RACE获得病毒基因组末端序列。序列测定与分析的结果表明,China/Tib/07株全长15948bp,预测编码6种结构蛋白和2种非结构蛋白,与已发表小反刍兽疫病毒基因组的长度和结构相似;在系统进化上与西南亚流行毒株有很高的同源性(91.6%～98.1%);与麻疹病毒属的其它成员相比,与牛瘟病毒的同源性最高(64.3%)。

Peste des petits ruminants virus is a member of Morbillivirus Paramyxoviridae.The complete genome of a Peste des petits ruminants virus（PPRV）isolate,China/Tib/07 was sequenced and molecular characteristics was analyzed.The internal sequences of the virus genome were amplified by RT-PCR with primers designed according to the published data in GenBank,while the sequences of the 3＇ and 5＇ ends of the genome were determined by RACE.Amplification products were directly sequenced,assembled and analyzed with DNAStar4.0.Results showed that China/Tib/07 genome consisted of 15 948 nucleotides in length,encoding six structural proteins and two non-structural proteins just like other known PPRV genomes.Phylogenetically,the virus genome shared homology of 91.6%~98.1% with Southwest Asian isolates among PPRV strains and the highest homology of 64.3% with rinderpest virus among morbillivirus members.