HPLC法测定射干合剂中多指标成分的含量

Determination of multiple-components in Shegan mixture by HPLC

目的建立能同时检测射干合剂样品中5种黄酮类化合物及麻黄碱含量的HPLC法。方法样品用超声提取法，用Inertsi PODS-3色谱柱分离样品，流动相为乙腈与1‰磷酸（pH=2．25），梯度洗脱，流量为1mL·min^-1，柱温为室温，用紫外检测仪215am测定;外标法定量。结果对于待测组分的r均〉0．998；黄芩苷、黄芩素、汉黄芩素、射干苷、次野鸢尾黄素、麻黄碱的线性范围均为0．05—50mg·L^-1.方法加样回收率为94．5％-103．2％；相对标准偏差（RSD）低于4．7％（n=3）。结论本法可用于射干合剂中多指标成分的含量测定，方法简便、准确、灵敏度高，也可用于制剂及体内指标成分的研究。

AIM To develop a method for the determination of ingredients of multiple indicator in Shegan mixture by RP-HPLC. METHODS Shegan mixture samples were extracted with selective ultrasound extraction. Separation and quantification were achieved by a Inertsil; ODS-3 column under the optimum conditions and acetonitrile 1‰ phosphoric acid aqueons （pH = 2.25） as mobile phase at a flow rate of 1 mL·min^- 1,at room temperature and detected with UV detector at 215 nm. RESULTS The quantified analysis of external standard calibration curves was used. The calibration graph was linear in the range of 0.05 - 50 mg· L^-1 with r 〉 0.998 for BCL, BCN, WGN, TRD, IRT and ED, respectively. For spiked sample analysis,the RSD was less than 4.7% and the recoveries were 94.5% - 103.2%. CONCLUSION This method is simple, accureate and convenient, and can be applied to the Shegan mixture samples for interventional studies as well as observational studies.