微波辅助萃取-高效液相色谱法测定金银花药材中槲皮素和木樨草素的含量

Study on microwave assisted extraction HPLC method for determination of two flavonoids compunds of Flos lonicerae

目的建立微波辅助萃取-高效液相色谱（MAE-HPIE）法分析金银花药材中槲皮素和木樨草素的含量。方法采用Diamomil C18柱（150mm×4．6mm，5μm），以甲醇-0．2％三氟乙酸（55：45，V／V）为流动相，流速1．0mL·min^-1，检测波长350nm。微波辅助萃取条件：2．5mol·L^-1盐酸甲醇溶液10mL，微波功率为360W，提取时间为3min。结果槲皮素在5-100mg·L^-1内线性关系良好（r=0．9995），平均加样回收率为92．35％，RSD为1．33％（n=3）；样品的日内和日间RSD分别为0．44％和1．61％。木樨草素在0．25～5mg·L^-1内线性关系良好（r=0．9999），平均加样回收率为92．40％，RSD为1．13％；日内和日间RSD为0．79％和2．60％。结论该方法快速、准确，操作便捷，适用于提取分析和测定金银花中槲皮素和木樨草素的含量。

AIM To develop a microwave assisted extraetion-HPLC method for the determination of two flavonoids eompunds of Flos lonicerae. METHODS Quereetin and luteolin in Flos lonicerae were determined by HPLC on Diamonsil C18 column （ 150 mm × 4.6 mm,5 μm） with methanol - 0.2% trifluoroaeetie acid （55：45, V/V）as the mobile phase. The detection wavelength was 350 nm. The flow rate was 1.0 mL· min^-1. Quereetin and luteolin were both extracted by microwave assisted extraction with 10 mL 2.5 mol·L^-1 methanol-ehlorhydrie acid using condition of 360 W and 3 rain. RESULTS The linear range of quereetin was 0.25 - 5 mg·L^-1 （ r = 0.999 9） and the linear range of luteolin was 5 - 100 mg·L^-1 （ r = 0. 999 5）. The average recovery and RSD value of quereetin were 92.35% and 1.33% （ n = 3） ; the average recovery and RSD value of luteolin were 92.40% and 1.13% （ n = 3）, respectively. The RSD of intra-day and inter-day of quereetin were 0.44% and 1.61%, respectively; the RSD of intra-day and inter-day of luteolin were 0. 79% and 2.60%, respectively. CONCLUSION The method is simple, precise and accurate, and ean be used to determine and analyze quereetin and luteolin in Flos lonicerae.