SH2-B对结肠癌HT-29细胞增殖的影响

SH2-B enhances proliferation of colon cancer HT-29 cells

目的探讨SH2-B对结肠癌HT-29细胞周期演进和细胞增殖的影响。方法免疫荧光筛选SH2-B低表达结肠癌细胞(HT-29)。用LipofectAMINE2000将pcDNA3.1-SH2-B质粒转染至HT-29细胞(转染组),并设空载体转染组和未转染组作为对照。Western-blotting分析SH2-B转染后SH2-B的表达,MTT法分析SH2-B转染后HT-29细胞的增殖,流式细胞术分析SH2-B转染后HT-29细胞周期变化。结果将pcDNA3.1-SH2-B质粒转染至HT-29细胞后可获得SH2-B的有效表达。转染SH2-B后HT-29细胞增殖显著增强(P<0.05)。流式细胞仪检测结果显示,转染SH2-B组S期细胞显著高于未转染组和空载体组(P<0.05)。结论 SH2-B促进结肠癌HT-29细胞增殖和细胞周期演进。

Objective To investigate the effect of SH2-B on proliferation and cell cycle of colon caner HT-29 cells.Methods Screening of the SH2-B low expression colon cancer cell line（HT-29） by immunofluorescence assay was done.pcDNA3.1-SH2-B was transfected into HT-29 cells using LipofecTAMINE 2000.After gene transfection,SH2-B expression was detected by Western-blotting,cell proliferation was measured by MTT assay,and cell cycle was analyzed by flow cytometer.Results Immunofluorescence assay results showed that SH2-B lowly expressed in HT-29 cells.after being transfected with pcDNA3.1-SH2-B,SH2-B expression dramatically increased in HT-29 cells,and HT-29 cells proliferation was significantly enhanced（P0.05）.Cell cycle analysis showed that S stage cells were significantly increased in the SH2-B transfected group（39.4±2.31） % compared with the blank control group（18.6±4.25）% and vector control group（11.1±2.39）%（P0.05）.Conclusions SH2-B may enhance colon cancer HT-29 cell proliferation and promote cell cycle.