黄芩苷对内毒素作用的人牙周膜细胞增殖和分泌肿瘤坏死因子-α的影响

Effects of scutellarin on the proliferation and expression of tumor necrosis factor-α in human periodontal ligament cells treated with LPS

目的:观察野黄芩苷对内毒素脂多糖(LPS)作用的人牙周膜细胞增殖和分泌肿瘤坏死因子-α的影响。方法:原代培养人牙周膜细胞,采用MTT比色法观察野黄芩苷对LPS作用的人牙周膜细胞增殖活性的影响,采用酶联免疫方法检测培养上清液中的TNF-α水平。结果:100μg/ml LPS可抑制人牙周膜细胞的增殖活性、刺激人牙周膜细胞分泌肿瘤坏死因子-α。加入LPS同时分别给予0.001～10μg/ml不同浓度野黄芩苷,能促进人牙周膜细胞的增殖活性并能抑制LPS刺激人牙周膜细胞肿瘤坏死因子-α的分泌,其中在1μg/ml时作用达到最强。结论:野黄芩苷对LPS作用的人牙周膜细胞具有保护作用。

Objective： To investigate the effects of scutellarin on the proliferation and expression of tumor necrosis factor-α（TNF-α） in human periodontal ligament cells（HPDLCs） treated with lipopolysaccharide（LPS）.Methods： Human periodontal ligament cells（HPDLCs） were primarily cultured in vitro.MTT colorimetric assay was performed to assess the cell proliferation.Enzyme-Linked immunosorbent assay（ELISA）was applied to detect the level of TNF-α in the culture supernatant.Results： The proliferation ability was remarkably inhibited by incubation of HPDLCs with 100 μg/ml LPS of E.Coli,while the level of TNF-α was significantly increased.After adding different concentration of scutellarin from 0.001 to 10 μg/ml,the proliferation activity was greatly improved and the production of TNF-α was inhibited,with the optimal effect at 1 μg/ml of scutellarin.Conclusion： Scutellarin may have protective effects on human periodontal ligament cells treated with LPS.