人胚胎干细胞培养与肺再生

Human embryonic stem cells culture and lung regeneration

背景:肺研究领域,利用人胚胎干细胞在体外增敏培养基已成功生成Ⅱ型肺泡细胞。由于体外实验中表现出不定向增殖潜能,胚胎干细胞有望成为可应用于移植、基因治疗等取之不尽的细胞源。目的:综述人胚胎干细胞培养与肺再生的研究进展。方法:应用计算机检索2000-01/2010-01中国知网数据库相关文章,检索词为"人胚胎干细胞,肺组织再生",并限定文章语言种类为中文。同时计算机检索1990-01/2010-01PubMed数据库相关文章,检索词为"Human Embryonic stem cells,Lung regeneration",并限定文章语言种类为English。共检索到文献396篇。结果与结论:由于肺组织结构复杂、再生能力有限,肺疾病的治疗已成为棘手的问题。目前认为人胚胎干细胞能定向分化成为肺组织细胞,将人胚胎性干细胞植入损伤的肺组织,通过诱导剂使其向肺泡上皮细胞分化,从而产生肺泡组织。这是一种很有前途的治疗手段,但目前尚处于研究的初级阶段。

BACKGROUND：In the field of pulmonary research,studies using human embryonic stem cells have succeeded in generating enriched cultures of type II pneumocytes in vitro.On account of their potential of indefinite proliferation in vitro,embryonic stem cells could be a source of an unlimited supply of cells that are available for transplantation and for use in gene therapy.OBJECTIVE：To review recent advance on human embryonic stem cell culture and lung regeneration.METHODS：We retrieved related literatures of China National Knowledge Infrastructure with the key words of ＂human embryonic stem cells,lung regeneration＂ from January 2000 to January 2010 in Chinese by computer.We then retrieved related literature of PubMed with the key words of ＂human embryonic stem cells,lung regeneration＂ from January 1990 to January 2010 in English by computer.A total of 396 articles were searched.RESULTS AND CONCLUSION：Because lung tissues have a complex structure and little capacity of regeneration,it is difficult to cure for lung diseases.At present,it is known that human embryonic stem cells are able to differentiate into lung tissue cells directionally;human embryonic stem cells are engrafted into the injured lung tissues,and induced to differentiate into alveolar epithelial cells,and further develop alveolar tissue.This is a promised therapeutic tool,but it remains in the basal research stage.