姜黄煎剂对内皮细胞组织损伤大鼠丝裂素活化蛋白激酶、肿瘤坏死因子α和白细胞介素6的影响

Effects of curcuma decoction on mitogen-activated protein kinase, tumor necrosis factor alpha and interleukin-6 in rats with vascular endothelial injury

背景:已证实姜黄素有广泛的抗炎作用,对血管内皮有保护功能。目的:观察姜黄煎剂对内皮细胞组织损伤模型大鼠丝裂素活化蛋白激酶及肿瘤坏死因子α和白细胞介素6的影响,从炎性反应的角度,在分子水平探讨姜黄煎剂保护血管内皮细胞的机制。方法:将Wistar大鼠随机平均分为正常对照组,假手术组,模型组和姜黄煎组。模型组与姜黄煎组均复制血管内皮组织损伤模型鼠。姜黄煎组在造模24h后开始用姜黄煎(100mg/kg)灌胃,模型组给予生理盐水(3mL/d)灌胃,连续14d,1次/d。假手术组开腹后即缝合,不插入球囊导管。所有动物均于术后3,7和14d取材,各组血管组织内皮样品均应用ELISA法检测血清中丝裂素活化蛋白激酶与肿瘤坏死因子α和白细胞介素6水平含量,并用苏木精-伊红染色法观察急性损伤后大鼠血管内膜组织结构的变化。结果与结论:动脉内膜组织损伤后的3,7和14d中姜黄煎组血清丝裂素活化蛋白激酶,肿瘤坏死因子α和白细胞介素6水平明显低于模型组和假手术组(P<0.05),以14d最为显著(P<0.05)。血管内膜损伤7d后姜黄煎组内膜组织增生明显降低(P<0.05)。由此可知,姜黄煎干扰可有效下调血管内膜组织损伤后大鼠模型血清丝裂素活化蛋白激酶、肿瘤坏死因子α和白细胞介素6的表达,对内皮细胞具有保护作用,其作用机制可能与抑制肿瘤坏死因子α和白细胞介素6的表达,阻断丝裂素活化蛋白激酶的信号传导有关。

BACKGROUND： Previous studies demonstrated that curcumin has widely anti-inflammatory effect and protects vascular endothelial cell against injury. OBJECTIVE： To observe the effects of curcuma decoction on mitogen-activated protein kinase （MAPK）, tumor necrosis factor α （TNF-α） and interleukin-6 in rats with vascular endothelial injury, and to explore the molecular mechanism of protection effects from the aspect of inflammatory reaction. METHODS： Wistar rats were randomly divided equally into normal control, sham operation, model and curcuma groups. Rats were prepared for vascular endothelial injury models in the model and curcuma groups. At 24 hours after model preparation, rats in the curcuma group were intragastric administrated 100 mg/kg curcuma decoction, once per day, for 14 successive days. Rats in the model group were intragastric administrated 3 mL/d physiological saline. There was laparotomy suture without balloon catheter insertion in the sham operation group. All animals were sacrificed at 3, 7 and 14 days after operation. The expressions of MAPK, TNF-α and interleukin-6 were measured by ELISA assay. The morphological changes of vascular endothelial cells were observed by hematoxylin-eosin staining. RESULTS AND CONCLUSION： The expressions of MAPK, TNF-α and interleukin-6 in the curcuma group were significantly lower than those in the model and sham operation groups at 3, 7 and 14 days after operation （P〈0.05）, especially at 14 days （P〈0.05）. Compared with other groups, the endometrial hyperplasia in the curcuma group was decreased at 7 days after operation （P〈0.05）. The results showed that curcuma interference protects vascular endothelial cell via inhabiting TNF-α, interleukin-6 expressions and blocking MAPK signals.