高氧液对大鼠胰腺移植的保护作用

Protective effect of hyperoxic fluid on pancreas transplantation in rats

背景:目前可供移植的器官日益短缺,但因灌注冷保存所至的原发性移植物无功能仍然有一定的发生率,减少灌注保存所至的器官损伤有较大的临床意义。目的:探讨高氧液对大鼠胰腺移植的保护作用。方法:40只SPF级Wistar大鼠,随机分成2组(n=20),高氧液组灌注高氧液,对照组灌注普通林格液,比较两组灌注后胰尾组织电镜结果、移植后第1天、3天血血糖浓度,以及两组移植后胰腺组织病理急性排斥评分。结果与结论:移植前两组差异无显著性意义,灌注后高氧液组胰腺组织超微结构基本正常,细胞核形态完整,大量线粒体存在;对照组胰腺组织细胞大部分排列仍有序整齐,胞浆内线粒体水肿明显,部分线粒体空泡变性。移植后第1,3天血血糖高氧液组低于对照组(P<0.05);高氧液组评分低于对照组(P<0.05)。可见高氧液可减轻缺血再灌注损伤,对大鼠胰腺移植有一定的保护作用。

BACKGROUND： Organ for transplantation is insufficient, and primary transplantation of nonfunction caused by perfusion cryopreservation occasionally occurs. It has clinically significant to reduce organ damage caused by perfusion preservation. OBJECTIVE： To study the protective effect of hyperoxic perfusion fluid on pancrease transplantation in rats. METHODS： Totally 40 Wistar rats were randomly divided two groups and poured with natrium lacticum ringer’s solution or hyperoxic natrium lacticum ringer’s solution. After perfusion, the tail pancreases of rats were checked by electron microscopy. The glucose concentrations and acute rejection score of pancreas tissues were compared between two groups at 1 and 3 days after pancreas transplantation. RESULTS AND CONCLUSION： There was no statistics difference between two groups prior to operation. The ultrastructure of pancreatic tissue was basically normal, with completely nuclei and plenty of mitochondria in the hyperoxia liquid group after perfusion. The pancreatic tissues still orderly arranged in the control group, with obviously swelling in mitochondria, and parts of mitochondria were vacuolar degenerated. The glucose concentrations of the hyperoxia liquid group were lower than that of the control group at 1 and 3 days after transplantation （P 0.05）. The acute rejection scores of the hyperoxia liquid group were lowerthan that of the control group （P 0.05）. Thus, hyperoxia liquid can mitigate the ischemia reperfusion injury and protect pancreastransplantation in rats.