HLA-A位点PCR-SSP基因分型和血清学分型的比较

A comparison between PCR SSP genotyping and serotyping for HLA A locus

目的：建立优化ＨＬＡＡ位点ＰＣＲＳＳＰ分型方法。方法：采用普通扩增仪和Ｅｐｐｅｎｄｏｒｆ管对细胞系ＤＮＡ和３７个健康正常人进行基因分型，血清学检测采用标准淋巴细胞毒试验方法。结果：发现引物浓度和浓度比、ＤＮＡ的纯度及酶的选用，是影响ＨＬＡＡ位点ＰＣＲＳＳＰ分型准确性的重要因素。该方法对３７个标本的基因分型结果与血清学结果相符合。结论：ＰＣＲＳＳＰ方法具有简便准确的优点。

Objective:To establish experimental method of PCR SSP for HLA A locus genotyping.Methods:Genomic DNA from 37 non related healthy individuals were tested by using ordinary PCR amplifier and Eppendorf tubes.Serological typing was conducted using standard microcytotoxicity method.Results:It was found that the concentrations and ratios of primers,the purity of DNA sample and the Taq polymerase employed seemed to be crucial for the accuracy of the HLA A locus PCR SSP.The genotyping results obtained with this method for 37 Hans coincide exactly with the serological results.Conclusion:The PCR SSP method for the HLA A locus is apparently a good alternative to the serological typing and could be spread for HLA A typing.