摘要
目的:探讨Bcl2在系统性红斑狼疮(SLE)发病机制中作用。方法:采用流式细胞仪双标记法检测31例SLE病人外周血T、B细胞Bcl2蛋白表达。结果:发现活动期SLE病人CD3+、CD4+和CD8+T细胞Bcl2蛋白表达明显高于非活动期SLE病人、其他疾病组和正常对照组。CD19+B细胞Bcl2蛋白表达在各组之间并无统计学差异。CD3+T细胞Bcl2蛋白表达的平均荧光强度(MFI)与SLE疾病活动指数(SLEDAI)成正相关关系(r=0.8195,P<001),而与血沉、补体C3、C4水平及自身抗体(ANA、抗dsDNA、抗Sm)无相关。
Objective:In order to investigate Bcl 2 expression in the pathogenesis of Systemic lupus erythematosus(SLE).Methods:Tested Bcl 2 protein levels in T,B cells of 31 patients with SLE by two colourcytofluorography.Results:Compared with inactive SLE patients,normal controls and other non SLE patients,a proposition of T cells (including CD3 +,CD4 + and CD8 + subgroups) expressed Bcl 2 protein increased significantly in active SLE patients.However,Bcl 2 protein levels did not statistically differentiate in CD19 + B cells among all groups.Mean fluorescence intensity(MFI) of Bcl 2 protein on CD3 + T cells,which wasn't related to serological indices,was positively related to the SLE disease activity index(SLEDA) among SLE patients.Conclusion:Abnormal expression of Bcl 2 in T cells might play an important role in the active stage of SLE patients.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第2期85-86,共2页
Chinese Journal of Immunology
基金
国家自然科学基金