系统性红斑狼疮病人血T、B淋巴细胞Bcl-2的表达

Expression of Bcl 2 of peripheral blood T,B lymphocytes in patients with systemic lupus erythematosus

目的：探讨Ｂｃｌ２在系统性红斑狼疮（ＳＬＥ）发病机制中作用。方法：采用流式细胞仪双标记法检测３１例ＳＬＥ病人外周血Ｔ、Ｂ细胞Ｂｃｌ２蛋白表达。结果：发现活动期ＳＬＥ病人ＣＤ３＋、ＣＤ４＋和ＣＤ８＋Ｔ细胞Ｂｃｌ２蛋白表达明显高于非活动期ＳＬＥ病人、其他疾病组和正常对照组。ＣＤ１９＋Ｂ细胞Ｂｃｌ２蛋白表达在各组之间并无统计学差异。ＣＤ３＋Ｔ细胞Ｂｃｌ２蛋白表达的平均荧光强度（ＭＦＩ）与ＳＬＥ疾病活动指数（ＳＬＥＤＡＩ）成正相关关系（ｒ＝０．８１９５，Ｐ＜００１），而与血沉、补体Ｃ３、Ｃ４水平及自身抗体（ＡＮＡ、抗ｄｓＤＮＡ、抗Ｓｍ）无相关。

Objective:In order to investigate Bcl 2 expression in the pathogenesis of Systemic lupus erythematosus(SLE).Methods:Tested Bcl 2 protein levels in T,B cells of 31 patients with SLE by two colourcytofluorography.Results:Compared with inactive SLE patients,normal controls and other non SLE patients,a proposition of T cells (including CD3 +,CD4 + and CD8 + subgroups) expressed Bcl 2 protein increased significantly in active SLE patients.However,Bcl 2 protein levels did not statistically differentiate in CD19 + B cells among all groups.Mean fluorescence intensity(MFI) of Bcl 2 protein on CD3 + T cells,which wasn't related to serological indices,was positively related to the SLE disease activity index(SLEDA) among SLE patients.Conclusion:Abnormal expression of Bcl 2 in T cells might play an important role in the active stage of SLE patients.