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CCCTC-结合因子与核周蛋白α4存在分子间相互作用并调控其基因表达 被引量：2

CCCTC-Binding Factor Interacts with Karyopherin Alpha 4 and Regulates its Gene Expression
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摘要 目的:研究CCCTC-结合因子(CTCF)是否与核周蛋白α4(KPNA4)有蛋白间相互作用并调控其表达。方法:用GSTpull-down实验研究CTCF是否与KPNA4存在蛋白间相互作用;用逆转录半定量PCR和免疫印迹检测CTCF敲降对KPNA4基因表达的影响。结果:GSTpull-down实验表明CTCF与KPNA4之间存在相互作用;当CTCF敲降时,KPNA4基因的表达随之下降。结论:CTCF与KPNA4之间存在相互作用并调控其基因表达。 Objective： To research whether CCCTC-binding factor（CTCF） interact with karyopherin α4（KPNA4） and regulate its gene expression. Methods Results： GST pull-down experiment results showed that there＇s a protein-protein interaction between CTCF and KPNA4. The reverse transcription semi-quantitative PCR and immunoblotting assay results showed when CTCF was knocked down, the expression level of KPNA4 gene was reduced. Conclusion： CTCF interacts with KPNA4 and regulates its gene expression.
作者 沈晶晶 黄培堂 朱旭东
机构地区 军事医学科学院生物工程研究所
出处 《生物技术通讯》 CAS 2010年第4期481-483,共3页 Letters in Biotechnology
基金 艾滋病和病毒性肝炎等重大传染病防治国家重大专项(2008ZX10002-021)
关键词 CCCTC-结合因子 核周蛋白α4 基因表达调控 蛋白质相互作用 CCCTC-binding factor karyopherin α4 gene expression regulation protein-protein interaction
分类号 Q75 [生物学—分子生物学]
  • 相关文献

参考文献6

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二级参考文献15

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共引文献5

同被引文献11

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生物技术通讯
2010年 第4期

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