摘要
目的:研究CCCTC-结合因子(CTCF)是否与核周蛋白α4(KPNA4)有蛋白间相互作用并调控其表达。方法:用GSTpull-down实验研究CTCF是否与KPNA4存在蛋白间相互作用;用逆转录半定量PCR和免疫印迹检测CTCF敲降对KPNA4基因表达的影响。结果:GSTpull-down实验表明CTCF与KPNA4之间存在相互作用;当CTCF敲降时,KPNA4基因的表达随之下降。结论:CTCF与KPNA4之间存在相互作用并调控其基因表达。
Objective: To research whether CCCTC-binding factor(CTCF) interact with karyopherin α4(KPNA4) and regulate its gene expression. Methods Results: GST pull-down experiment results showed that there's a protein-protein interaction between CTCF and KPNA4. The reverse transcription semi-quantitative PCR and immunoblotting assay results showed when CTCF was knocked down, the expression level of KPNA4 gene was reduced. Conclusion: CTCF interacts with KPNA4 and regulates its gene expression.
出处
《生物技术通讯》
CAS
2010年第4期481-483,共3页
Letters in Biotechnology
基金
艾滋病和病毒性肝炎等重大传染病防治国家重大专项(2008ZX10002-021)