用含10%胎牛血清的DMEM培养肠出血性大肠杆菌O157∶H7可增强其对HeLa细胞的黏附/擦拭损伤

Attaching and Effacing Lesions Caused by EHEC O157∶H7 is Greater if Bacterium Cultured in DMEM(10% FBS)

目的:研究生长在不同培养基中的肠出血性大肠杆菌(EHEC)O157∶H7对宿主细胞造成的黏附/擦拭损伤是否存在差异。方法:分别用LB、DMEM、DMEM(含10%胎牛血清)、DMEM(含终浓度为25mmol/L的HEPES)等4种培养基培养O157∶H7,然后感染HeLa细胞,利用Giemsa染色观察细菌黏附差异,进行肌动蛋白荧光染色实验并观察宿主细胞肌动蛋白聚集差异。结果:在含10%胎牛血清的DMEM培养基中培养EHECO157∶H7,其黏附力增加,聚集细胞骨架肌动蛋白的能力明显增强。结论:为进一步研究EHECO157∶H7的致病性,探索O157∶H7新的致病因子奠定了基础。

Objective： To explore that attaching and effacing lesions is different when EHEC O157∶H7 were grown in different culture. Methods： O157∶H7 grown in LB, DMEM, DMEM（10% FBS）, DMEM（25 mmol / L HEPES） were allowed to adhere to monolayers of HeLa cells. The infected monolayers were stained with Giemsa solution to explore the adherence. Also, the infected monolayers were analysis by fluorescence actin staining to observe the actin accumulation. Results： DMEM（10% FBS） remarkably stimulate bacterial adherence. And, grown in DMEM （10% FBS）, O157∶H7 assembled cytoskeletal actin more efficiently. Conclusion： The research results will have an important value for the further research on phathogenesis of EHEC O157∶H7.