应用反转录骤合酶链反应研究庚型肝炎病毒感染

StudyofHepatitisGVirusInfectionbyReverse-TranscriptionPolymeraseChainReaction

建立反转录聚合酶链反应（ＲＴ－ＰＣＲ）检测庚型肝炎病毒感染的方法。该法特异性强，检测下限（１．９×１０３）与感染剂量相当，简便实用。对我国各类人群的ＨＧＶ感染情况进行研究，结果表明，慢性丙型肝炎患者、抗－ＨＣＶ阳性职业献血员和已知肝炎病毒标志物均阴性者感染率较高，分别为６０％、４０％和２１．７％。对其中一株（Ｐ１１）阳性扩增产物所做部分核酸序列分析，与国外两株相应区段的同源性高达９６．４％和９５．３％。

Abstract Reverse-transcriptionpolymerasechainreaction(RT-PCR)forthedetectionofhepatitisGvirus(HGV)RNAwasestablishedThismethodisspecific,sensitive(Athresholdsensitivityof19103viralgenomecopyequivalentspermililliterofstartingspecimen,equallingtotheinfectiondose),simpleandapplicablePrimarystudyshownthattheinfectionrateofHGVwerehigheramongthepopulationofchronichepatitisC(60%),non-AtoEhapatitis(40%)andprofessionaldonorswithanti-HCVantibodies(217%)Thenucleicacidsequencesofoneclone(p11)wascomparedwiththatofHGU44402andHGU45966andtheidentityratesare964%and953%respectively