肝癌患者细胞因子分泌和肿瘤浸润淋巴细胞杀伤肝癌细胞的研究

The secretion of cytokine from patients with hepatocellular carcinoma and observasion of TIL kills cancer cells under electronic microscope

为探讨肝癌肿瘤浸润淋巴细胞（ＴＩＬ）杀伤肝癌细胞的作用机制，用透射电镜观察了ＴＩＬ与肝癌细胞共同培养后，ＴＩＬ吸附、包绕和吞噬自体和ＥＢＬ－７４０２肝癌细胞，促使肝癌细胞凋亡。接受ＴＩＬ治疗的肝癌术后患者，外周血单个核细胞（ＰＢＭＣ）产生干扰素（ＩＦＮγ）活性和血清肿瘤坏死因子（ＴＮＦ－α）活性，明显高于手术对照组。与单纯手术治疗组比较，ＴＩＬ组生存时间明显延长。肝癌术后应用ＴＩＬ的疗效显著优于单纯手术治疗，可望为肝癌的综合治疗提供新的方法。

Tumour-infiltrating lymphocytes(TIL) were isolated from 19 patients with hepatocellular carcinoma.After TIL treatment,the patients secrete activity of interferon (IFN-) and tomour necrosis factor (INF-) were higher then the controls.The feature of TIL adhering to cancer cells was observed under electron microscope in our experiment.The density of chromosome of autologous cancer cells and 7402 hepatocellulare cancer cells was increased.Then membrane of cell and mitochondria in cancer cells were partly breaked.The results has shown that antitomour activity of TIL is related to hepatocellular cancer cells apoptosis with production cytokine of the patients.