利用后巩膜加固术治疗家兔近视眼后成纤维细胞生物力学性能变化

Biomechanical properties of fibroblasts after posterior scleral reinforcement treatment for rabbit experimental myopia

目的研究后巩膜加固术后家兔眼球不同时期成纤维细胞力学特性的变化,从生物力学的角度探讨后巩膜加固术的作用机制。方法 3周龄新西兰家兔45只随机选取一侧眼球用眼睑缝合方法制备近视动物模型,建模60天后,眼球随机分为两组,A组行后巩膜加固术(PSR),B组行相似手术(不放置加固条带)。分别于术后3个月和6个月取材培养培养巩膜加固区及过渡组织的成纤维细胞。用免疫细胞化学法对培养的细胞进行鉴定。利用微管吸吮方法结合半无限体细胞力学模型测定各组成纤维细胞的力学特性(包括成纤维细胞的平衡弹性模量E肄和表观黏性μ)。结果经统计学分析,两组巩膜成纤维细胞的平衡弹性模量及表观黏性均无统计学意义(P>0.05)。后巩膜加固术6个月,经统计学分析,两组巩膜成纤维细胞的平衡弹性模量及表观黏性均无统计学意义(P>0.05)。后巩膜加固术后3月组与6月组的巩膜成纤维细胞比较,E肄和μ的差异均无统计学意义(P>0.05)。后巩膜加固术后3个月与6个月组的过渡区成纤维细胞的力学特性,E肄分别为(289.2依84.3),(276.9依113.9)Pa;μ分别为(1575.2依459)Pa·s,(1492.2依562.6)Pa·s,其差异均无统计学意义(P>0.05)。结论后巩膜加固术的增强作用并不是原位巩膜成纤维细胞本身生物力学的加强,而是由于加固条带及引起过渡区的存在。

Objective To evaluate the biomechanical properties of fibroblasts for rabbit experimental myopia after Posterior Scleral Reinforcement（PSR） treatment,and discuss the mechanism of PSR in myopia treatment as viewed from biomechanics.Method 45 rabbits of three-week-old were randomly monocular treated by eyelid suture to prepare experimental myopia eye.After 60 days,the experimental myopia eyes were divided into two groups randomly.Group A was treated by PSR.Group B was treated by similar operation without placing reinforce strap.After three months and six month,the fibroblasts from each group were isolated and cultured in vitro respectively.The cultured cells were then determined to be fibroblasts by using immunocyte chemistry method.Micropipette aspiration technique was used to investigate the viscoelastic properties of the fibroblasts from each group with mechanical model of semi-infinite somatic cells.Results Three months after operation,the viscoelastic properties of the scleral fibroblasts in Group A and Group B exhibit no significant difference（P0.05） three months and six months as well（P0.05） after operation with the equilibrium modulus,E∞,and apparent viscosity,μ of the scleral fibroblasts in Group A（E =（361.2± 121.1）Pa、μ=（2928.2±669.4）Pa·s） compared with that in Group B（E =（347.6± 82.1）Pa、μ=（2820.6± 593.5）Pa·s）.Neither in Group A nor Group B,the E∞ and μ at different stages after operation have significant difference（P0.05）.The E∞ and μ in transition zone tissues at different stages after operation have no significant difference（P0.05） either.Conclusions The enhancement of PSR is caused by transition zone tissues and the strip itself.