重症肌无力患者IL-18、TGFβ-1的表达

Expression and clinical significance of IL-18 and TGFβ-1in patients with myasthenia gravis

目的探讨白细胞介素18(interleukin-18,IL-18)、转化生长因子β-1(transforming growth factorβ-1,TGFβ-1)在重症肌无力发病机制中的作用。方法采用基因芯片技术对30例重症肌无力患者和30名正常人外周血单个核细胞TGFβ-1、IL-18mRNA表达水平进行分析,采用酶联免疫吸附试验的方法检测血清IL-18、TGFβ-1水平,对重症肌无力患者病情按临床绝对评分法进行评分,并比较其临床意义。结果 MG患者外周血清IL-18、TGFβ-1水平显著高于正常组水平(t=6.18,P<0.01),MG患者基因芯片检测IL-18Ratio值为2.026,TGFβ-1Ratio值为2.34,结果具有一致性,Ⅱ型、Ⅲ型MG患者IL-18水平高于Ⅰ型患者,与患者临床绝对评分呈正相关(r=0.827,P<0.05)。结论 IL-18、TGFβ-1均参与重症肌无力发病机制,IL-18血清水平可能与重症肌无力病情轻重程度相关。

Objective To study the pathogenic mechanism and clinical significance of Transforming growth factor β-1 （ TGFI3-1 ） and interleukin-18 （ IL-18 ） in myasthenia gravis. Methods Differential expression of TGFβ-1 ,IL-18 mRNA in 30 patients with myasthenia gravis（MG） and 50 normal controls （NC） were analyzed using Biostar Human-6-V3 type hu- man genes of eDNA microarray. The serum levels of TGFβ-1 and IL-18 were measured by enzyme-linked immunosorbent as- say （ELISA）. The absolute scores of 30 MG patients were rated using XU Xian-hao score system. Results The levels of TGFβ-1 and IL-18 were significantly higher in MG than in NC group （P 〈 0. 01 ）, which was consistent with the result of cDNA microarray. The levels of IL-18 were higper in MG patients of Type Ⅱ and Type m than in MG patients of Type I. The levels of IL-18 were positively correlated with the absolute scores of MG, Conclusions IL-18 and TGFβ-1 are involved in the pathogenic immunologic mechanisms of MG, and IL-18 might be associated with the severity of MG.