氯乙烯对雄性大鼠生殖内分泌激素的影响

Effect of vinyl chloride on reproductive and endocrine system of male rats

目的 探讨氯乙烯对雄性大鼠生殖内分泌激素的影响.方法 雄性SD大鼠60只,随机分为对照组和10、100和1000mg/kg氯乙烯染毒组,每组15只,腹腔注射染毒14和28d.测定大鼠血清中睾酮（T）、卵泡刺激素（FSH）、黄体生成素（LH）、雌二醇（E2）、抑制素B（InhB）和睾丸中E2、T、InhB的水平,并观察睾丸支持细胞和间质细胞超微结构的改变.结果 与对照组比较,染毒14 d各染毒组大鼠血清中T和E2水平降低,InhB和LH水平升高,仅100 mg/kg染毒组LH水平差异有统计学意义（P〈0.05）.染毒28 d,100、1000 mg/kg染毒组大鼠血清中T分别为（10.90±1.56）和（8.52±2.85）ng/ml,InhB水平分别为（31.40±6.21）和（28.39±5.67）pg/ml,均明显低于对照组[T为（15.89±4.03）ng/ml,lnhB为（46.87±8.74）pg/ml],差异有统计学意义（P〈0.05）;各染毒组大鼠血清中FSH水平均明显高于对照组.差异有统计学意义（P〈0.05）.与染毒14d比较,染毒28d各染毒组大鼠血清中InhB和LH水平均明显降低,差异有统计学意义（P〈0.05）.染毒28 d时100、1000mg/kg染毒组大鼠睾丸组织中T和InhB水平分别为（8.05±2.19）、（6.75±1.94）ng/mg pro和（39.32±5.55）、（35.53±8.71）pg/mg pro,与对照组[T为（11.90±2.33）ng/mg pro、InhB为（49.45±7.01）pg/mg pro]比较,差异有统计学意义（P〈0.05）.超微结构观察可见,染毒组的睾丸间质细胞和支持细胞出现了细胞核畸形、线粒体肿胀等改变.结论 氯乙烯对雄性大鼠生殖内分泌系统有损害作用,可引起生殖内分泌激素水平改变和睾丸间质细胞、支持细胞超微结构变化.

Objective To explore the effect of vinyl chloride on reproductive and endocrine system of male rats. Methods Male SD rats were administered with vinyl chloride at dose of （0,10,100,1000 mg/kg） for 14 and 28 days, respectively. The levels of testosterone（T）,inhibin B, luteinizing hormone（LH）, follicle stimulating hormone （FSH） and estradiol（E2） were measured in serum and testis homogenates. Histopathologi-cal examinations were performed for testis with electron microscopy. Results Compared with the control group after 14-day exposure, T and E2 serum levels of 1000,100,10 mg/kg groups decreased, InhB and LH levels of three dose groups increased. LH serum levels of 100 mg/kg increased significantly statistically compared with control group （P〈0.05）. After 28-day exposure, T serum levels of 100,1000 mg/kg groups were （10.90±1.56）, （8.52±2.85） ng/ml respectively （P〈0.05）, InhB serum levels of 100,1000 mg/kg groups were （31.40±6.21）, （28.39±5.67） pg/ml respectively. Both of T and InhB serum levels of 100,1000 mg/kg groups decreased significantly （P〈0.05）. Serum FSH levels of 10,100,1000 mg/kg groups decreased significantly compared with control group（P〈0.05）.Compared with groups of 14-day exposure, serum InhB and LH levels of 10,100,1000 mg/kg groups decreased significantly statistically after 28 days. T and InhB testis levels of 100,1000 mg/kg groups were 8.05±2.19）, （6.75±1.94） ng/mg pro and （39.32±5.55）, （35.53±8.71 ）pg/mg pro respectively, which decreased significantly compared with control group（P〈0.05）. Leydig cell and Sertoli cell were damaged according to histopathological examinations. Conclusion Vinyl chloride has adverse effects on reproductive and endocrine system of male rats and may change their serum and testis homogenate levels of hormones.