绝经后女性头发变化与雌激素受体β基因多态性的关系

Association of Female Hair Changes after Menopause with Estrogen Receptor β Gene Polymorphism

目的探讨绝经后女性头发生长特征的变化,对其与雌激素受体β基因多态性的关系进行分析。方法选择健康中老年绝经后女性作为受试对象,使用毛发检测仪对顶部和颞部受试区头发进行拍照,图像分析软件计算头发生长速度、密度和白发率;同时采集受试者抗凝静脉血,应用聚合酶链反应-限制性片段长度多态性技术,对受试人群的雌激素受体β基因RsaⅠ和AluⅠ酶切多态性进行分析。结果 131名绝经后女性基因型频率分布符合Hardy-Weinberg平衡定律(P>0.05)。受试者顶部和颞部白发率与RsaⅠ酶切位点有相关性,差异有显著性(P=0.040,P=0.008)。头发密度、生长速度在各组间有一定差异,但无显著性(P>0.05)。结论雌激素受体β基因RsaⅠ酶切多态性与绝经后女性白发的发生有关。

Objective To investigate the hair changes in post-menopausal women, and analyse its relationship with estrogen receptor β gene polymorphism. Methods Healthy post-menopausal women were recruited, images of the areas in vertical and temporal region were obtained using hair detector; hair growth rate, hair density, white hair rate were counted by image analyzing software. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique was employed to determine estrogen receptor β Rsa I and Alu I gene polymorphism. Results The distribution of genotypes was in Hardy-Weinberg equilibrium （P 〉 0.05）. The differences of white hair rate were statistically significant in Rsa I polymorphism. Hair growth rate, hair density had no significance among different subgenotypes. Conclusion The Rsa I polymorphism of estrogen receptor β is associated with white hair growth in post-menopausal women.