苦瓜自交系多糖结构组成的比较分析

Comparative Analysis of Polysaccharides Structure and Composition in Bitter Melon Inbreed Lines

对苦瓜3个自交系果实中的多糖结构和组成进行了比较研究。采用热水浸提乙醇沉淀法得到苦瓜粗多糖,进一步经过脱蛋白、透析等纯化工艺得到苦瓜多糖,应用气相色谱法测定其多糖组成,红外光谱法测定其糖苷键类型。研究结果表明:在同一生长环境下,3个苦瓜自交系多糖在其含量、单糖组成以及结构上均存在差异;苦瓜水溶性多糖主要由阿拉伯糖、甘露糖、葡萄糖和半乳糖组成,此外3号和8号自交系还含有少量的鼠李糖和木糖;构型分析表明苦瓜多糖主要以α型和β型吡喃糖形式存在。

The polysaccharides structure and composition of fruit in three bitter melon inbred lines were compared in the paper.The crude polysaccharides were extracted in hot water and precipitated with alcohol.Then,the polysacchrides were purified by the processes of protein removing and dialysis.The polysaccharides composition and the types of its glycosidic bond were determined by gas chromatography and infrared spectroscopy respectively.The results showed that in the same growing environment,there are significant differences in the polysaccharide content,monosaccharide composition and the structure among different bitter melon inbreed lines.The water-soluble polysaccharide of bitter melon is mainly composed of arabinose,mannose,glucose and galactose,with the exception that inbreed lines of No.3 and No.8 contain small amount of rhamnose and xylose.In addition,configuration analysis showed the polysaccharides mainly exist in the forms of α-and β-pyranose in bitter melon.