益肾胶囊对糖尿病肾病大鼠肾组织炎症因子NF-κB的影响

Effect of Yishen Capsule on Expression of NF-κB in Renal Tissue of Rat with Diabetic Nephropathy

目的:观察益肾胶囊对糖尿病肾病(DN)大鼠肾组织核转录因子-κB(nuclear factor-κB,NF-κB)表达的影响。方法:将40只健康雄性Wistar大鼠随机分为:正常对照组(对照组)、DN模型组(模型组)、氯沙坦组、益肾胶囊组,每组10只。利用链脲佐菌素(STZ)诱导右肾切除的大鼠制备DN模型。氯沙坦组灌胃氯沙坦钾20mg·kg-1.d-1,益肾胶囊组灌胃益肾胶囊625mg·kg-1.d-1,对照组及模型组每日给予等量的蒸镏水灌胃,实验周期为12周。实验过程中观察大鼠24h尿蛋白定量、血肌酐(Scr)、尿素氮(BUN)变化,光镜下观察肾脏病理变化,采用免疫荧光法检测各组大鼠肾组织NF-κB的表达。结果:12周末,DN模型组大鼠的24h尿蛋白定量、Scr、BUN均高于正常对照组(P<0.05),肾组织中NF-κB表达水平明显高于正常对照组(P<0.05);益肾胶囊治疗组大鼠24h尿蛋白定量、Scr、BUN均低于模型组(P<0.05),肾组织NF-κB表达水平明显低于DN模型组(P<0.05)。结论:益肾胶囊可能通过下调DN大鼠肾脏组织NF-κB的表达,延缓DN的进展。

Objective：To observe the yishen capsule of diabetic nephropathy （DN） of renal inflammatory factor NF-κB （nuclear factor-κB） expression,DN for the yishen capsule provides theoretical and experimental basis.Methods：40 healthy male Wistar rats were randomly divided into：normal control group （control group）,DN model group （model group）,losartan group,yishen capsule group,10 in each group.Using streptozotocin （STZ） induced rat right yishen removed preparation DN model.Losartan losartan potassium administered daily 20 mg·kg-1·d-1,yishen capsule administered daily yishen capsule 625 mg·kg-1·d-1,the control group and model group every day for the same amount of distilled pour stomach,experimental period of 12 weeks.Experiment rats 24 h urinary protein,serum creatinine （Scr）,blood urea nitrogen （BUN） changes in light of renal pathological changes were observed using immunofluorescence in renal tissue NF-κB expression.Results：12 weekends,DN model group,24 h urinary protein,Scr,BUN were higher than the control group （P0.05）,renal tissue expression of NF-κB was significantly higher than the normal control group （P0.05）;yishen capsule treated rats 24 h urinary protein,Scr,BUN were lower than model group （P0.05）,renal tissue expression of NF-κB levels were significantly lower than the DN model group （P0.05）.Conclusion：yishen capsule in rats may be attributed to yishen DN NF-κB expression,delaying the progress of DN.